摘要
目的探讨转化生长因子-β1(TGF-β1)在原发性肾病综合征患者血清及肾组织中的表达情况及其相关性。方法采用常规病理、免疫组织化学方法对肾组织进行染色,并经病理图像分析系统进行分析;血TGF-β1水平采用酶联免疫吸附法测定。结果肾病组患者的血清中TGF-β1的含量明显高于正常对照组(P<0.01);TGF-β1在正常对照组肾脏仅有少量表达,而在肾病组患者肾小球及肾小管中有较密集的棕黄色颗粒,图像分析仪计算显示肾病组肾组织中的TGF-β1表达较正常对照组显著增多,差异具有统计学意义(P<0.01);肾病组血清中TGF-β1表达与肾组织中的表达呈正相关(P<0.01)。结论肾病综合征患者血清及肾组织TGF-β1表达显著增加,在肾脏纤维化过程中发挥重要作用。
Objective To investigate the transforming growth factor-β1（TGF-β1） in serum of patients with primary nephrotic syndrome and renal tissues and its relevance.Methods General pathology,immunohistochemistry staining of kidney tissue,and pathological image analysis system;blood levels of TGF-β1 enzyme-linked immunosorbent assay.Results Nephropathy in patients with serum levels of TGF-β1 was significantly higher than that of the control group（P0.01）;TGF-β1 in the normal control group,only a small amount of renal expression,and in kidney disease patients the glomerular and tubular a more dense brown granules,image analysis calculations show that nephropathy in renal tissue expression of TGF-β1 than the control group was significantly increased,the difference was statistically significant（P0.01）;nephropathy serum TGF-β1 expression and kidney tissues was positively correlated（P0.01）.Conclusions Patients with nephrotic syndrome and renal expression of TGF-β1 significantly increased in renal fibrosis play an important role.
出处
《医药论坛杂志》
2011年第18期59-60,共2页
Journal of Medical Forum
关键词
肾病综合征
转化生长因子-Β1
肾组织
免疫组化
Nephrotic syndrome
Transforming growth factor-β1
kidney
Immunohistochemistry
