摘要
目的观察肾康灵对TGF-β1干预后肾小管上皮细胞内E-cadherin、α-SMA表达,探讨其抗肾间质纤维化机制。方法采用Western bloting法、RP-PCR法观察空白组、TGF-β1组、肾康灵组的α-SMA、E-cadherin的表达。实验结果采用SPSS 23.0进行统计。结果肾康灵可下调α-SMA、上调E-cadherin m RNA的表达水平,对比TGF-β1组,差异具有统计学意义(P<0.05)。结论肾康灵通过下调α-SMA,上调E-cadherin的水平,缓解肾间质纤维化。
Objective Investigate the expression of the α-SMA and E-cadherin in the rat renal tubular epithelial cell induced in TGF-β1 by shenkangling, in order to clarify the possible mechanism in preventing renal interstitial fibrosis. Methods The expression of α-SMA by Western blotting and detect E-cadherin m RNA by RT-PCR in control group, TGF-β1 group and shenkangling group was measured. All the data were analyzed by SPSS 23.0. Results Compared with TGF-β1 group, the expression of α-SMA in shenkangling group was down-regulated, and E-cadherin was up-regulated（P〈0.05）. Conclusion Shenkangling can reduce renal interstitial fibrosis by decrease the level of α-SMA and increase the level of E-cadherin.
作者
卢小露
艾斯
郑健
LU Xiaolu1,3, AI Si1,3, ZHENG Jian1,2,3(1. Pediatrics Department, People＇s Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou Fujian 350004, China; 2. Traditional Chinese Medicine College, Fujian University of Traditional Chinese Medicine, Fuzhou Fujian 350122, China; 3. Fujian Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Kidney Diseases, Euzhou Fujian 350004, China)
出处
《中国卫生标准管理》
2018年第18期117-119,共3页
China Health Standard Management
