摘要
以广昌“太空莲 3 6号”子藕上的顶芽或侧芽为外植体 ,接种于不同激素配比的MS培养基上诱导不定芽 ,然后将不定芽转入较高激素配比的继代培养基上进行快速繁殖。试验结果表明 :芽分化最佳培养基为MS +6-BA 1 .5～ 2 .0mg/L +NAA 0 .0 5mg/L+0 .2 %活性炭 ,快速增殖培养基以MS +6-BA 0 .5mg/L +NAA 0 .0 5mg/L +KT 0 .
The buds of Guangchang white lotus (Nelumbo nucifera Gnertn)were used as explants and incubated on eight kinds of MS medium containing different hormone combinations for the differentiation of buds. The differential buds were transferred to several subcultural media for inducing adventitious buds as well as their rapid propagation.The research results showed that the best differentiation medium for the inducement of buds was MS+6-BA 1.5～2.0mg/L+NAA 0.05mg/L+0.2% active carbon,the best subcultural medium was MS+6-BA 0.5mg/L+KT 0.2mg/L+NAA 0.05mg/L.\;
出处
《江西农业学报》
CAS
2001年第4期58-61,共4页
Acta Agriculturae Jiangxi
关键词
广昌太空莲
激素配比
组织培养
快速繁殖
培养基
Hormone combination
Tissue culture
Rapid propagation
Guangchang white lotus
