摘要
白细胞介素-6(interleukin-6,IL-6)是当代细胞因子研究中倍受分子生物学家和免疫学家关注的生物活性肽。已经证明IL-6不仅对免疫效应细胞(B细胞、T细胞、巨噬细胞、天然杀伤细胞和淋巴因子激活杀伤细胞)
Large scale preparation of IL-6 mRNA was obtained from humanspleen cells stimulated with PHA+TPA at the peak of IL-6 mRNA trans-cription. Full length of IL-6 mRNA was translated in wheat germ ex-tract to produce bioactive IL-6 (tIL-6), the IL-6 mRNA was about 18 Sdepending on the 7 mol/L urea-SDS-PAGE, recovery of the RNA fromthe gel and translation in vitro. The elute curve of tIL-6 was the sameas recombinant IL-6 (rIL-6) and natural IL-6 (nIL-6), the molecularweight of IL-6 was about 20000 Dalton. The translational process ofIL-6 mRNA in vitro was inhibited when RNA was cultured with RNaseor translated product was cultured with proteinase, proving a standardin vitro translation. The tIL-6 acts on MH60. BSF-2 cell line to supportthe IL-6-dependent cell proliferating, on SKW6-CL_4 cell line or SAC-activated B lymphoblast cell for secreting IgM, and binds with IL-6receptor of IL-6 response cell in IL-6 recoptor absorption assay.
出处
《生物工程学报》
CAS
CSCD
北大核心
1991年第3期275-278,共4页
Chinese Journal of Biotechnology
基金
国家自然科学基金
关键词
白细胞介素-6
信使核糖核酸
富集
Large-scale preparation
characterization
in vitro translation
human interleukin-6 mRNA
