摘要
应用ＲＴ－ＰＣＲ技术，从人的外周血单个核细胞中扩增出人ＩＬ－６受体的特异性ｃＤＮＡ片段，经琼脂糖电泳证实扩增片段大约为１４００碱基对。运用ＤＮＡ重组技术，将获得的片段连接到ＰＵＣ１８质粒中，经转化、筛选、酶切鉴定构建出重组质粒ｐＵＣＩＬ－６受体；经序列分析证实为编码人ＩＬ－６受体的ｃＤＮＡ片段。
Using RT-PCR and DNA recombniation techniques,we had amplified the fragment of IL-6R cDNA from PBMC of normal human.Electrophoresis of 1% agarose showed that the bard is about 1400 base pairs.By DNA sequence analysis,it was identified that the PCR product DNA fragment is exactly belonged to IL-6R DNA fragrnent.We linked the fragment to the plasmid pUC18,and obtained a recombinant of human IL-6R,named pUCIL-6R. By using this recombinant cDNA as a probe,Northern blotting with RNA of U266 cells and RNA of Jurkat cells were performed respectively.The result showed that there was mRNA of IL-6R,in U266 cells,but not in Jurkat cells.
出处
《天津医科大学学报》
1996年第3期5-6,13,共3页
Journal of Tianjin Medical University
