摘要
在对Toll样受体 4(TLR4)B细胞优势表位预测的基础上 ,合成该B细胞表位多肽 ,并以此为抗原免疫BABL/C小鼠 ,经鼠 鼠杂交 ,ELISA筛选 ,有限稀释克隆化 ,得到一株分泌抗TLR4合成肽的杂交瘤细胞株B4,腹水效价为 1∶10 0 0 0 0。其免疫球蛋白为IgG2 ,k型。ELISA鉴定表明该抗体能识别TLR4+ 性细胞 ,Westernblot分析显示在分子量约 90kD处呈现单一免疫色带。
An immunogenic peptide sequence of B cell dominant epitope of human Toll like receptor 4 (TLR4) was selected basing on predication of B cell epitope of TLR4.bALB/c mice were immunized with synthetic peptide of TLR 4 and one clone (B4) of hybridoma cells was picked out by ELISA and limited dilution. High titer McAb prepasations against TLR 4 were obtained from ascites fluid of BALB/c mice infravenously inoculated with the McAb inducing hybridoma cells.The titer of ascites fluid was 1∶ 100 000 .The subclass of the McAb was IgG2,k.The specificity of McAb was identified. by indirect ELISA,and a single strong blot was shown in molecular weights of 90 Kd.using Western blot analysis.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第1期11-12,共2页
Medical Journal of Chinese People's Liberation Army
基金
国家重点基础研究发展规划项目 (编号G19990 5 42 0 3)
全军医药卫生科研基金 (编号 0 1Q10 5 )资助课题
