摘要
目的 :研究准分子激光原位角膜磨镶术 (laserinsitukeratomileusis ,LASIK)角膜瓣复位后的黏附机制。方法 :实验组 2 4只新西兰兔分为 8组 ,每组 3只 ;对照组 3只。左眼按近视 - 10 .0 0D行LASIK术。分别于术后即刻、2 4h、1周、2周、1月、3月、6月及 12月行裂隙灯显微镜检查 ,并取角膜行光镜观察及免疫组织化学染色 ,检测细胞外基质 (extracellularmatrix ,ECM )成分 ,包括I型胶原、Ⅲ型胶原、Ⅵ型胶原、纤维连接蛋白 (cellularfibronec tion ,cFN)、细胞黏合素 (tenascin ,TN)、Ⅳ型胶原及层黏连蛋白 (laminin ,LN)。结果 :裂隙灯显微镜检查术后角膜瓣贴附紧密 ;光镜观察角膜瓣交界面早期存在缝隙 ,晚期贴附紧密 ;免疫组织化学检测角膜瓣交界面术后早期有cFN的明显表达及Ⅲ型胶原、Ⅵ型胶原的表达 ,无I型胶原、Ⅳ型胶原、LN及TN的表达。结论 :LASIK术后早期 ,角膜瓣与其下的基质层之间的黏附由FN、Ⅵ型胶原及Ⅲ型胶原完成。
Objective: To investigate the mechanism of corneal flap attached to the stromal bed after laser in situ keratomileusis(LASIK). Methods: 24 adult New Zealand White rabbits received LASIK in the left eye for -10.00 D ablation. at once, 24 hours, 1 weeks, 2 weeks, 1 months, 3 months, 6 months and 12 months after surgery, the corneas were examined periodically by slip lamp microscopy, and the left eyes of 3 rabbits were enucleated, the corneas were studies with light microscopy; immunohistochemical evaluation for extracellular matrix; including collagens type Ⅰ, type Ⅲ, type Ⅳ, type Ⅵ, fibronection, tenascin and laminin. Results: The flap attached to the stromal bed tightly after LASIK by slip lamp microscopy examination. For light microscopy examination ,the interface between the flap and the attached stroma had space in the early stage and adhered tightly in the later period; The interface had a large amount of fibronectin expression, and also had the expression of collagens type Ⅲ and type Ⅵ. Conclusion: The flap attached to the stroma bed tightly after LASIK without suture. This was correlated with the function of fibronection ,collagen type Ⅲ and type Ⅵ.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2001年第6期545-547,共3页
Journal of Peking University:Health Sciences
基金
高等学校博士学科点专项科研基金 ( 9119)~~
