摘要
目的 探讨Ⅲ期鼻咽分化型非角化性鳞癌中p5 3蛋白的表达与生物学行为及预后的关系 ,以及p5 3蛋白表达与p5 3基因突变和潜伏膜蛋白 (LMP) 1蛋白表达的关系。方法 应用免疫组织化学EnVison法和聚合酶链反应 单链构象多态性分析 (PCR SSCP)检测 5 8例Ⅲ期鼻咽分化型非角化性鳞癌标本。结果 5 8份Ⅲ期鼻咽分化型非角化性鳞癌中 ,p5 3蛋白总阳性率为 6 5 5 %(38/5 8) ,生存期在 5年以下的患者阳性率为 82 .1%(32 /39) ,5年以上患者 31.6 %(6 /19) ,两组间p5 3蛋白表达差异有显著性意义 (P <0 .0 5 ) ;p5 3蛋白表达在有颅底侵袭组为 74.4%(2 9/39) ,无颅底侵袭组为47 4 %(9/19) ,两组间差异有显著性意义 (P <0 .0 5 )。PCR SSCP检测 15例p5 3蛋白阳性的病例 ,p5 3基因 5～ 8外显子突变率为 0 (0 /15 )。LMP 1蛋白免疫组织化学总阳性率为 72 .4%(4 2 /5 8) ,与p5 3蛋白表达呈正相关关系 (r=0 .5 0 4)。结论 本组Ⅲ期鼻咽分化型非角化性鳞癌患者中p5 3蛋白表达与p5 3基因突变无相关关系 ,与LMP 1蛋白的表达相关。p5 3蛋白的表达阳性率随着患者生存期的延长而降低 ,有颅底侵袭组p5 3蛋白表达阳性率高于无颅底侵袭组。
Objective To investigate p53 protein expression at stage Ⅲ nasopharyngeal differentiated non-keratinizing squamous carcinoma (DNKSC) and their correlation with biological behavior and prognosis;the relationship between p53 protein expression and p53 gene mutation and LMP-1 protein expression at 58 cases of stage Ⅲ DNKSC. Methods Immunohistochemistry (Envision) was applied to detect p53 protein and LMP-1 protein expression. Non-isotopic PCR-SSCP was used to detect p53 gene mutation. Results The p53 protein expression showed general positive rates of 65.5% (38/58) in the patients with nasopharyngeal DNKSC, being significantly higher in the group surviving less than 5 years 82.1% (32/39) than in the group surviving longer than 5 years 31.6% (6/19) (P<0.05). Besides, it was closely correlated with the local invasion to the cranial base, there was a significant difference between invasion 74.4% (29/39) and non-invasion 47.4%(9/19) (P<0.05). The expression of LMP-1 protein rated 72.4% (42/58), exhibiting a positive correlation with the expression of p53 protein (r=0.504). The p53 gene mutation was found in none of the cases (0/15). Conclusions In stage III nasopharyngeal DNKSC, the expression of p53 protein is not related to p53 gene mutation, whereas it relates to the expression of LMP-1 protein. Declined expression of p53 protein is closely related to the time of survival, and the p53 protein expression is higher in the invasive carcinoma.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2001年第6期456-459,共4页
Chinese Journal of Pathology
基金
贵州省科委基金资助项目 (D98 3)
关键词
鼻咽肿瘤
基因
p53
蛋白质
预后
Nasopharyngeal neoplasms
Genes, p53
Protein p53
