摘要
目的 研究肝细胞生长因子 (hepatocyte growth factor,HGF)对视网膜色素上皮 (retinalpigment epitheliaum,RPE)细胞的促增生和促移行作用。 方法 在无血清培养液培养的人 RPE细胞中分别加入 1、2、10、5 0、10 0 μg/ L 不同浓度的 HGF,四唑盐比色法 (methyl thiazolyl tetrazolium,MTT)测定促细胞增生情况 ;采用 RPE细胞损伤愈合模型 ,在无血清培养液中分别加入 1、2、10、5 0、10 0μg/ L不同浓度的 HGF,培养 2 0 h后计数进入刮痕区的细胞 ,观察 HGF对 RPE细胞移行的作用。结果 HGF浓度处于10～ 10 0 μg/ L 时对 RPE细胞具有促增生作用 (P<0 .0 5或 P<0 .0 1) ,增生率为 18.2 %～ 34 .8% ,其中浓度为 5 0 μg/ L 作用 3d达到最大促增生效果 (P<0 .0 1) ,增生率为 32 .8% ;HGF可明显促进 RPE细胞移行 ,促移行能力分别为 113.0 % (10μg/ L ) ,91.7% (5 0μg/ L )和 5 0 .3% (10 0μg/ L )。浓度自 2μg/ L开始出现促细胞移行作用 (P<0 .0 5 ) ,促移行能力为 9.3%。结论 HGF可促进 RPE细胞增生和移行 ,是RPE细胞的有丝分裂原和强力的促移行因子。
Objective To investigate the effect of HGF on proliferation and migration in cultured human RPE cells. Methods Human RPE cells cultured in serum-free medium were treated with HGF(1,2,10,50,100 μg/L), and MTT assay was used to detect the growth of the cells; an in vitro wound healing model was used to count the number of cells that had entered the denudate area in RPE migration treated with HGF (1,2,10,50,100 μg/L) after 20 h. Results HGF(10,50,100 μg/L) increased proliferation rates of cultured human RPE (18 2 % to 34 8 %), and at a concentration of 50 μg/L on day 3 HGF induced the maximal increase of proliferation( P <0 01); HGF showed effects on migration of 9 3 %, 113 0 %, 91 7 % and 50 3 % at the concentrations of 2,10,50, 100 μg/L, respectively. HGF stimulated the strongest migratory response in RPE cells at a concentration of 10 μg/L (113 %). Conclusion HGF can induce the proliferation and obvious migration of RPE cells, consequently HGF was a mitogen and potent migratory factor for human cultured RPE cells.
出处
《中华眼底病杂志》
CAS
CSCD
2001年第4期307-310,共4页
Chinese Journal of Ocular Fundus Diseases
