摘要
目的 体外观察阿司匹林 (ASA)对胃癌细胞株SGC 790 1细胞增殖的影响 ,并探讨其可能的作用机制。方法 采用噻唑蓝 (MTT)法、流式细胞仪 (FCM )、电镜和3H TdR核素标记等技术 ,研究ASA对SGC 790 1细胞增殖的抑制和可能的机制。结果MTT显示体外ASA对SGC 790 1有细胞毒作用 ,与浓度和作用时间有相关性 ,3H TdR实验表明 ,ASA对细胞DNA合成有抑制作用。FCM显示 ,DNA直方图上出现典型的亚二倍体“凋亡峰” ,凋亡率在 7.8%～ 3 4 .4 %。使S期、G2 /M期细胞比例升高 ,G1期比例下降 ,呈一定剂量效应关系。电镜下见典型的细胞凋亡形态学特征 :细胞核染色质致密浓缩 ,凋亡小体形成等。结论 体外ASA对SGC 790 1细胞增殖有抑制作用 ,可能与诱导细胞凋亡和阻止细胞周期的进展有关。
Objective To study the effect of aspirin on cell proliferation of human gastric cancer cell line SGC-7901 and the probab le mechanism involved. Methods Using MTT method, flow cytometr y (FCM),~~~3 H-TdR incorporation a nd electron microscopy, the effect of aspirin on the SGC-7901 of proliferation and related mechanism were studied. Results Results showed that th e growth of SGC -7901 was inhibited by aspirin. The inhibitory rates for growth of SGC-7901 we re positively correlated with the concentration and duration of aspirin in the m edia. ASA could inhibit DNA syntheses. Sub-G_1 pe ak was detected by FCM, and the rate of apoptosis was between 7.8% and (34.4)% . The cell ratios o f S and G_2/M increased, whereas the cell ratio of G_0/G_1 phase decreased (after) treatment and was in a dose-dependent manner. The SGC-7901 cell line exhibited some mor p hologic features of apoptosis, including cell shrinkage, nuclear condensation, D NA fragmentation, and formation of apoptotic bodies under electron microscopy. Conclusions Aspirin inhibited the growth of human gastric cancer ce ll line SGC-7901. The effects of aspirin on the phase of cell cycle and inducing apoptosis may be partially explained as the cytotoxic effects of aspirin.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2001年第12期732-734,共3页
Chinese Journal of Digestion
