摘要
目的 研究肝细胞肝癌与癌旁肝组织胰岛素受体密度与亲和力的差异。方法 采用体外受体结合实验 ,通过 Scatchard分析 ,计算两种细胞膜的最大结合容量 (Bmax)与平衡解离常数 (Kd值 )。结果 肝癌细胞膜胰岛素受体 Bmax为 1.94± 0 .6 4pm ol/ mg蛋白 ,Kd值为 2 .12± 0 .6 2 nmol/ L;癌旁肝组织细胞膜胰岛素受体 Bmax为1.42± 0 .5 7pmol/ mg蛋白 ,Kd值为 2 .2 1± 0 .78nm ol/ L。肝癌细胞膜胰岛素受体 Bmax显著高于癌旁组织细胞膜 (P<0 .0 5 ) ,而肝细胞肝癌与癌旁肝组织细胞膜的胰岛素受体亲和力无显著性差异 (P>0 .0 5 )。结论 肝细胞肝癌较癌旁肝组织表达胰岛素受体增高 。
Objective To investigate the expression of insulin receptor in human hepatocellular carcinoma and it's adjacent tissue.Methods The human hepatocellular carcinoma and it's adjacent tissue specimens were obtained from 12 patients with histologically confirmed hepatocellular carcinoma at surgery and were immediately frozen under -80℃. Insulin was radioiodinated using Ch T method. Cell membrane fractions of human hepatocellular carcinoma and it's adjacent tissue were isolated by sucrose density gradient centrifugation. Receptor binding of 125 I insulin to human hepatocellular carcinoma and it's adjacent tissue were performed. Binding data were calculated according to Scatchard using the ligand program. Statistical comparison was made with the paired t test.Results The Kd values of human hepatocellular carcinoma and it's adjacent tissue were 2.12±0.62nmol/L and 2 21±0.78 nmol/L respectively; the values of B max were 1.94±0.64 pmol/mg protein and 1.42±0.57 pmol/mg protein respectively. The B max value of human hepatocellular carcinoma was significantly higher than that of the adjacent tissue ( P <0.05), whereas the two Kd values had little difference ( P >0.05).Conclusion The results of this study showed that human hepatocellular carcinoma expressed denser insulin receptors than it's adjacent tissue, but there was no significant increase in the affinity of the carcinoma to insulin.
出处
《华西医科大学学报》
CSCD
北大核心
2002年第1期32-34,共3页
Journal of West China University of Medical Sciences
基金
国家自然科学基金资助 (批准号 39770 2 2 9
