摘要
利用HPLC法对 2 84份种子硫代葡萄糖甙组成分析表明 ,新疆野生油菜依据种子中特征硫代葡萄糖甙组成可划分为 2类 ,91.2 %的新疆野生油菜种子主要含羟基苄硫甙 ,8.8%的新疆野生油菜种子主要含丙烯基硫甙 ,与黑芥和芥菜型油菜特征硫代葡萄糖甙组成一致。RAPD分析将新疆野生油菜、野芥和黑芥划分为 3簇 ,表明新疆野生油菜、野芥和黑芥之间存在明显的遗传距离 ,相对而言 ,新疆野生油菜与野芥间的遗传距离较近 ,与黑芥的遗传距离较远。新疆野生油菜可依据RAPD聚类分析结果划分为 4簇 ,其中第 1、2和 4簇主要由来源于新疆塔城地区的新疆野生油菜组成 ,种子主要含丙烯基硫甙 ,第 3簇为新疆巩留及其周边地区的新疆野生油菜 ,种子主要含羟基苄硫甙。
It was suggested that Xinjiang wild rape was the wild progenitor of Brassica nigra and Chinese Brassica juncea , to verify this hypothesis seed glucosinolate profile and RAPD were employed to reveal the genetic relationship among Chinese Xinjiang wild rape, Sinapis arvensis and Brassica nigra . Seed glucosinolates of 284 Chinese Xinjiang wild rapes were determined by HPLC method, 259 wild rapes (91.2%) were found containing sinalbin as the major glucosinolate in seeds, 25 wild rapes (8.8%) were characterized with dominant sinigrin, which was identical to Brassica nigra and Chinese Brassica juncea . Xinjiang wild rape, Sinapis arvensis and Brassica nigra were grouped into 3 independent clusters based on cluster analysis of RAPDs, considerable genetic divergence was discovered among Xinjiang wild rapes, which was parallel with the change of glucosinolate profile, Xinjiang wild rape was more related to Sinapis arvensis than to Brassica nigra . The results provide new evidence for the origin of Chinese Brassica juncea .
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2001年第4期1-6,共6页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金重点项目 (3 983 0 2 70)
关键词
新疆
野生油菜
野芥
黑芥
硫代葡萄糖甙
遗传分化
系统演化
Xinjiang wild rape
Sinapis arvensis
Brassica nigra
RAPD
Glucosinolate
Genetic divergence
Phylogeny
