摘要
目的 建立一种改良固相致敏红细胞吸附技术 (SPASE) ,用于快速检测各种肝病患者血清中金属蛋白酶组织抑制因子 1 , 2 (TIMP 1 ,TIMP 2 ) ,了解血清TIMP 1和TIMP 2在各种肝病患者中的意义。方法 用TIMP 1和TIMP 2单克隆抗体分别包被微量血凝板 ,加热冲洗后加入待检血清标本 ,最后加入单克隆抗体致敏红细胞。用致敏红细胞作为指示系统 ,观察红细胞吸附状况来判定结果。结果 SPASE法全过程仅需要 2 5～ 3h ,共检测 6 5 4例肝病患者血清标本 ,TIMP 1和TIMP 2阳性率分别为急性肝炎组 1 7 3 9%、1 4 1 3 % ;慢性肝炎组 3 3 1 9%、2 7 88% ;肝硬化组 82 84%、6 7 1 6 % (P <0 0 0 1 )。结论 TIMP 1和TIMP 2表达的变化可作为肝纤维化较为有用的诊断指标 ,TIMP 1的诊断意义更大。SPASE法具有较高的敏感性、特异性和快速性 ,不需复杂的仪器设备 ,操作简单 ,尤适于医院及基层医疗单位。
Objective Solid-phase absorption to sensitized erythrocytes(SPASE) test is a new technique of solid-phase aggregation. Using SPASE for rapid detection of TIMP-1 and TIMP-2 in serum in various patients with liver diseases and discuss the significance of TIMP-1 and TIMP-2 in serum in various patients with liver diseases. Methods Using antisensitized erythrocytes as indicator to replace enzyme or isotope, the result is judged by observing the agglutination of erythrocytes. A microtitre plate is coated by anti-TIMP-1 and anti-TIMP-2 McAb. After heating and washing, the serum sample is added to the microtitre plate.The McAb sensitized erythrocytes in put into the microtitre plate lastly.Results The results showed that the whole detection process by SPASE required only 2.5～3 h, it is a specific method for detection of TIMP-1 and -2 in serum and it's very convenient. In addition, it requires only a small amount of reagent, no special apparatus are needed. In 654 serum samples of all, the serum TIMP-1 and -2 levels are 17.39%, 14.13% in the group of acute hepatitis; 33.19%, 27.88% in the chronic hepatitis; and 82.84%, 67.16% in the liver cirrhosis group, respectively( P <0.001).Conclusion The serum TIMP-1 and -2 levels are very useful in the assessment of liver fibrosis in chronic liver disease. SPASE is an ideal technique for rapid detection of TIMP-1 and -2 in the serum, particularly for rural medical units.
出处
《西安医科大学学报》
CAS
CSCD
北大核心
2001年第5期438-440,共3页
Journal of Xi'an Medical University(Chinese)
