摘要
在实验室条件下用鼠疫菌接种菊科、豆科和禾木科的某些植物 ,第 5d～ 31 d用 PCR技术从紫苜蓿、胡枝子、茵陈蒿的根及大豆的根、茎、叶中扩增到鼠疫菌特异 DNA片段。第 1 0 d和第 2 0 d从大豆根中分离到鼠疫菌 ,鼠疫菌的菌落形态发生了 RS变异 ,毒力有所增强 ,同时营养需求也发生了变化。用染菌植物喂养豚鼠 ,第 1 2 d用放射免疫沉淀法检测血清 ,鼠疫菌特异抗体滴度达 1∶ 32 0。
Some plants of Asteraceal, Compositae, Fabaceal, Papilionacea and Poaceae, Gramineae were inoculated under the laboratory condition. The specific amplified DNA bands were detected by PCR technique in the roots stems and leaves of Medocago stativa ,Lespeeleza bicolov and Artemisia capillaris after inoculation from 5th day to 31st day. The strain of Y.pestis was isolated in the root of Glycine max at the 10th day and 20th day after inoculation, respectively. The shape of the colonies of Y.pestis showed RS variation. And the virulence of the strain was gotten amplified. At the same time, the nutrition requirement changed too. The infected plants were used to feed guinea pig. The serum of the pigs was detected by RIP. The titer of the specific antibody was up to 1∶ 320. It is believed that some plants probably play roles as nature host and medium of spread of Y.pestis.
出处
《地方病通报》
2001年第4期1-4,共4页
Endemic Diseases Bulletin
基金
吉林省卫生厅青年科研基金资助项目 (98589)
关键词
植物
鼠疫菌
作用
Plant
Y.pestis
Function
