摘要
目的 :探讨骨形态发生蛋白与人胚胎骨发生及发育的关系。方法 :用免疫组化技术和图像分析系统研究了第 9～ 2 4周人胚胎股骨中骨形态发生蛋白的分布规律及胎龄性变化。结果 :第 9周胚胎股骨为软骨雏形 ,雏形的软骨膜、软骨基质及其两端的软骨细胞骨形态发生蛋白呈阳性反应。第 1 0～ 2 4周胚胎股骨中 ,骨形态发生蛋白阳性反应主要见于成骨细胞、骨膜内层细胞、新生骨细胞及骨基质中 ;破骨细胞及骨髓细胞骨形态发生蛋白也呈阳性反应。骺软骨为阴性 ,但其内的软骨管为阳性反应。图象分析测定表明 :人胚胎股骨内成骨细胞及骨小梁的平均灰度值随胎龄增加而逐渐下降 ,同一股骨内不同部位的骨小梁和成骨细胞的平均灰度值有所不同。结论
Objective:To explore the relationship between bone morphogenetic protein and development of human fetal bone.Mothods:The Immunohistochemical method was utilized to sutdy the distribution of bone morphogenetic protein in the femora of human fetus ranged from the 9th to the 24th gestation week.And the changes of bone morphogenetic protein immunoreactive intensity in femoral sections were measured by the computer image analysis system.Results:The femora of 9 week fetuses were cartilage models,the perichondrium and cartilage matrix and chondrocytes at 2 ends showed bone morphogenetic protein positive reactivity.In the femora of the 10th to the 24th week fetuses,the bone morphogenetic protein positive reactivity existed mainly in osteoblasts,the inner layer cells of periosteum,newly born osteocytes and bone matrix.Osteoclasts and the cells of bone marrow also exhibited bone morphogenetic protein positive reactivity.The chondroepiphysis were negtive while the cartilage canals in it were positive.The computer image analysis measure showed that the average grey value of bone morphogenetic protein positive reactivity in osteoblasts and trabeculae of femora decreased with the advancing of gestational age,the average grey value varied in trabuculae and osteoblasts in different regions in the same femur.Conclusion:Bone morphogenetic protein may be closely related with the genesis and development of human fetal bone.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2001年第5期444-449,共6页
Chinese Journal of Anatomy
