摘要
为研究红曲对体外培养大鼠成骨细胞BMP-2表达的影响,从而为治疗骨质疏松症开发出中药新药提供细胞学依据。取成年SD大鼠48只,随机将大鼠分为A组(空白组)、B组(倍美力组)、C组(普拉固组)、D、E、F组(红曲高、中、低剂量组)6组,按10ml.kg-1分别予以生理盐水,倍美力水溶液(0.00625mg.ml-1),普拉固水溶液0.2mg.ml-1)及红曲水提液(1.25g.ml-1、0.625g.ml-1、0.125g.ml-1)灌胃。灌胃10天后,腹腔静脉取血制备含药血清;将从胎鼠颅骨取材的成骨细胞培养于含药血清培养液中,然后应用BMP-2免疫组化染色方法观察红曲对体外培养成骨细胞BMP-2表达的影响。结果显示,通过免疫组化染色发现BMP-2棕色深染位于细胞浆,红曲高、中剂量组成骨细胞棕色深染数量明显高于空白组,低剂量组与空白组无明显差异,阳性细胞比例呈剂量依赖性增长。表明红曲含药血清可以呈剂量依赖性地促进成骨细胞BMP-2表达;在细胞和分子水平为中药红曲治疗骨质疏松症提供了客观化的依据。
The objective of the paper is to study the influence of Semen Oryzae cum Monasco(SOM) on BMP-2 expression of rat osteoblasts cultivated in vitro and thus to offer the cytological basis for the development of the new TCD for treating osteoporosis.Forty-eight SD adult rats were randomly divided into six groups(n=8 rats each) treated transgastrically according to 10 ml·kg: the first three —Group A as the blank treated by normal saline,Group B and Group C treated by Premarin(0.00625 ml1) and Pravachol(0.25mg· ml1) water solutions,respectively;and the rest three treated by SOM extract liquid—Group D,Group E and Group F treated by high-dose(1.25g· ml1),middle-dose(0.625g· ml1) and by low-dose(0.125g· ml1),respectively;after the treatment of 10 days,the drug-containing serum was prepared from the abdominal venous blood and the osteoblasts taken from the fetal-rat cranial bone were cultivated in the media with the drugcontaining serum and then the influence of SOM on the BMP-2 expression of rat osteoblasts cultivated in vitro was observed with the immunohistochemical staining method.The results showed that the deep brown staining of BMP-2 was located in the cytoplasm;the numbers of deep-brown-stained osteoblasts in Group D and Group E were obviously higher than the one of Group A but Group F had no significant difference from Group A;the ratio of positive osteoblasts were increased in the dependence on the drug dose,suggesting that the SOM-containing serum functions to promote the BMP-2 expression of osteoblasts in the dependence on the SOM dose,which provides an objective basis for the treatment of osteoporosis by SOM on the cellular and molecular level.
出处
《中医正骨》
2006年第5期5-6,共2页
The Journal of Traditional Chinese Orthopedics and Traumatology
基金
浙江省中医药管理局科研基金资助项目
编号2003-63
