摘要
目的研究吡格列酮对Aβ25-35所致痴呆大鼠学习记忆能力和海马内氧化应激反应的影响。方法侧脑室注射Aβ25-35建立大鼠AD模型,吡格列酮(40mg/kg、80mg/kg)灌胃21d,次日行Morris水迷宫实验,前5天定位航行训练,第6天进行空间探索实验。行为学实验后第2天麻醉,断头取脑,提取脑组织匀浆,以备ELISA实验。ELISA实验研究大鼠海马内的氧化应激反应。结果 Morris水迷宫结果显示,模型组大鼠潜伏期较空白对照组明显延长,而穿过平台次数和平台滞留时间缩短。经吡格列酮治疗后,潜伏期显著缩短,穿过平台次数和滞留时间也相应增加。ELISA结果显示,模型组大鼠海马内酶性抗氧化剂GSH-Px和SOD活性较空白对照组明显降低,而脂质过氧化产物MDA含量增加。经吡格列酮治疗后,GSH-Px和SOD活性提高,MDA产生减少。结论吡格列酮能改善痴呆大鼠的学习记忆能力,其机制可能与对抗海马内的氧化应激反应有关。
Objective To study the effects of pioglitazone（Pio）on learning and memory deficits and oxidative stress in the hippocampus of Aβ25-35 induced AD rats.Methods Intracerebroventricular injection of Aβ25-35 to produce the model of AD.And the rats were administrated with Pio（40,80mg/kg,ig.）for 21 days.Morris water maze test was used to test learning and memory ability of the rats.The first 5days,place navigation test was carried out followed by the spatial probe test on the sixth day.After behavior test,the rats were sacrificed for preparing tissue homogenate.And ELISA was used to measure the activity of GSH-Px and SOD and the level of MDA.Results Morris water maze test showed that the escape latency was prolonged in rats of model group compared to the control group while the number of crossing the platform and time spent on the platform were reduced.After administration of Pio,the escape latency was decreased and the number of crossing the platform and time spent on the platform were increased.Results of ELISA showed that Pio increased the activity of GSH-Px and SOD and decreased the level of MDA in the AD rats.Conclusions Pio could improve the learning and memory ability of AD rats and the mechanism maybe related to attenuating oxidative stress in the hippocampus.
出处
《贵州医药》
CAS
2016年第2期119-122,共4页
Guizhou Medical Journal
