摘要
实验模拟活体卵泡环境研究了猪卵母细胞体外自发成熟和次黄嘌啉 (HX)对体外自发成熟的影响 ,建立了猪卵母细胞体外成熟的无血清培养模型。结果表明 ,猪卵丘 卵母细胞复合体 (CEO)和裸卵母细胞 (DO)在M 199培养基中能够自发成熟 ;
In this study pig oocyte natural maturation and the effect of hypoxanthine(HX)on pig oocyte maturation were investigated,and a serum free pig oocyte culture model in vitro was established mimic follicular environment in vivo .The results demonstrated that pig cumulus cell enclosed oocyte(CEO)and denuded oocyte(DO)could resume meiosis spontaneously cultured in the serum free M 199 environment.Hypoxanthine(HX1～4mmol/L)can inhibit the spontaneous resumption dependent on the dose and this inhibitory action was reversible.
出处
《上海农业学报》
CSCD
2001年第4期99-101,共3页
Acta Agriculturae Shanghai
基金
上海市农业科学院青年科技基金资助项目
