摘要
在已知参数条件下 ,通过电场诱导酸橙 (Cit rusaurantiumL .)叶肉原生质体和沙漠蒂甜橙(C .sinensisOsbeckcv .Shamouti)的胚性愈伤组织原生质体融合 ,融合产物经培养再生出 40棵植株。染色体检查表明所得到的植株具有 3 6条染色体 ,为四倍体植株。再生植株具有翼叶 ,叶片厚 ,表现出多倍体的特征。采用 2个 1 0 碱基随机引物鉴别再生植株的杂种特性。在 2个引物的扩增带型中 ,再生植株的随机扩增带图里出现了融合亲本的特征带。对再生植株染色体计数和RAPD分析的结果表明它们是酸橙和甜橙种间异源四倍体体细胞杂种植株。这些体细胞杂种植株的获得为选择具有酸橙优良性状。
Leaf derived protoplasts of sour orange( Citrus aurantium L.) were electrically fused with embryogenic protoplasts of sweet orange( C. sinensis Osbeck cv. Shamouti) based on fusion parameters as previously reported. Forty plants have been obtained from fusion products. Chromosome counting showed that the plants were tetraploids with 36 chromosomes. The plants have thick petiole winged leaves, indicative of the characteristics of polyploid. Two 10 bp random primers were utilized to identify the hybridity of the plants. Bands specific to fusion parents were detected in the amplification band patterns of the regenerated hybrid plants for the primers. Chromosome counting and RAPD analysis demonstrated that interspecific allotetraploid somatic hybrid plants have been successfully recovered between sour orange and sweet orange. The regeneration of the somatic hybrid plants between sour orange and sweet orange provides good experimental materials for selecting novel rootstock possessing superior traits of sour orange coupled with CTV resistance.
基金
ThisworkissupportedbytheNationalNaturalScienceFoundationofChina( 39830 2 60 )andpartiallybyHubeiNaturalScienceFoundation( 2 0 0 0J113)
IFSgranttoDr.LIUJi Hong(D/30 0 1 1)
