摘要
目的 探讨中药白英提取物对肝癌BEL 74 0 4细胞系的凋亡作用。方法 用不同浓度的白英提取物处理肝癌BEL 74 0 4细胞 ,通过观察细胞形态、DNA凝胶电泳法研究白英提取物诱导肝癌BEL 74 0 4细胞产生凋亡现象。结果 光学显微镜下可见细胞呈凋亡特征性变化 ,凝胶电泳可见细胞DNA有规律断裂形成梯形图谱。结论 白英可诱导肝癌BEL 74 0
Aim To study the biological effect of extracts of solanum lyratum thumb on inducing apoptosis of BEL 7404 cell line. Methods The BEL 7404 cells were cultured in DMED medium and treated with extract of solanum lyratum thumb and then were observed with microscope and their DNA was assayed by the method of gel. Results Treatment by extract of solanum lyratum thumb could induce the cells to reveal a typical apoptotic appearance. Their DNA was fracted and a ladder pattern characteristic could be found by the electrophoresis. The extract of solanum lyratum thumb show the same effects as paclitaxol. Conclusion solanum lyratum thumb can induce apoptosis in BEL 7404 cells.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2001年第3期200-203,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金资助项目(№ 3 0 0 1161940 )
科技部科技攻关项目 (№ 96 90 1 0 2 0 9)
