摘要
１９９６－２０００年从不同来源的病鳖和病鲤体内共分离到四株产生棕色色素的细菌，经细菌常规鉴定法和编码鉴定法确定为温和气单胞菌。进一步对细菌的产生色素特性、毒力因子和致病性进行检测，结果显示：（１）四株细菌均能产生水溶性棕色色素，但在有氧培养条件下细菌产生色素的速度随培养基种类不同而不同，依次为普通琼脂平板＞肉汤培养基＞琼脂半固体高层管。而在厌氧培养条件下细菌不能产生色素。（２）四株细菌均能产生外毒素及胞外蛋白酶二种毒力因子，毒素溶血价为１：１６或１：３２。以四株细菌肉汤培养液对小白鼠及本种动物均有致死性，为致病性温和气单胞菌。
strains of Aeromonas Sobria producing pigments were isolated from diseased soft-shelled turtle, Trinyx sinensis and eels, Anguilla japonica from 1996 to 2000. The four strains were classified as Aeromonas Sobria by normal identification method and numerical identification method. In addition, the bacterial characteristics of producing pigments, virulent factors and pathogenicity were detected. The results showed: (1) The four strains could produce water soluble brown pigment,but in aerobic culture, the productive speed was varied with different medium, agar plate> bouillon> semi-solid agar tube respectively. While in anaerobic condition, they could not produce any pigment. (2) The four strains were all able to produce toxin and protease, and the titers of toxin were 1:16 or 1:32. (3) They were pathogenic, and Aeromonas Sobria was lethal to mouse. eels and soft-shelled turtle.
出处
《水产学报》
CAS
CSCD
北大核心
2001年第5期428-431,共4页
Journal of Fisheries of China
基金
安徽省教委科学基金资助项目(97JL062)
关键词
鳖
鳗鲡
温和气单胞菌
色素产生特性
毒力因子
致病性
Trinyx sinensis
Anguilla japonica
Aeromonas Sobria
characteristics of producing pigment
virulent factor
pathogenicity
