摘要
目的 :研究 2 3 羟基桦木酸在体外对B16、SGC 790 1、A0、HL 60、K5 62、HeLa及S180 7种恶性肿瘤细胞生长的抑制作用。方法 :应用四甲基偶氮唑盐比色分析、电镜观察、流式细胞术等方法观测 2 3 羟基桦木酸对 7种肿瘤细胞的抑制作用。结果 :10～ 160mg·L-1的 2 3 羟基桦木酸在体外对 7种肿瘤细胞有显著的抑制作用 ,呈一定的剂量依赖关系 ,并可引起大量肿瘤细胞凋亡。 2 3 羟基桦木酸 Ⅰ使B16肿瘤细胞被阻滞在G0 G1期 ,而 2 3 羟基桦木酸 Ⅱ则使细胞阻滞在G2 M期。结论 :2 3 羟基桦木酸对体外 7种恶性肿瘤细胞均有显著的抑制作用 ,并使细胞阻滞在某一生长期 ,最终导致肿瘤细胞凋亡。
Objective To study the growth inhibition in seven cancer cell lines induced by 23?hydroxyl betulinic acid in vitro .Methods 3?(4,5?dimethylthiazole?2?yl)?2,5?diphenyl?tetrazolium bromide(MTT) assay,electronic microscopy and flow cytometry were used to examine the effect of 23?hydroxyl betulinic acid on seven cancer cell lines.Results 23?hydroxyl betulinic acid at the dose of 10～160 mg·L -1 markedly inhibited the growth of seven cancer cell lines in dose dependent man ner,and finally cause a great number of cancer cells apoptosis.23?hydroxyl betulinic acid?Ⅰ result in accumulation of B16 cells by retarding cell cycle at G 0?G 1 phase and 23?hydroxyl betulinic acid?Ⅱ arresting S180 at G 2?M phase.Conclusions 23?hydroxyl betulinic acid significantly inhibited the proliferation of seven cancer cell lines by retarding cells at certain phase of cell cycle and finally caused cells apoptosis.
出处
《东南大学学报（医学版）》
CAS
2001年第3期141-144,共4页
Journal of Southeast University(Medical Science Edition)
基金
国家自然科学基金资助项目 (39770 898)
