摘要
从不同地区、不同鱼种的濒死病鱼的实质性脏器中分离到一种革兰氏阴性、有运动力的短杆菌,具有如下特点:氧化酶和V-P反应均呈阳性,发酵葡萄糖产酸、产气,发酵甘露醇、水杨素和阿拉伯糖,不发酵肌醇,鸟氨酸脱羧酶阴性,对新生霉素不敏感。用API-20E系统试剂盒检测,证实其为嗜水气单胞菌。用该菌人工感染健康鱼,可引致与自然发病鱼类似的病症,其半数致死量(LD_(50))为5×10~3个菌。在该菌的培养上清中存在着一种具有溶血活性的毒素。研究证明,家养鲤科鱼暴发性传染病的病原为产毒素的嗜水气单胞菌,该病的准确名称应为嗜水气单胞菌败血症。
Systemic investigations on the pathogen of epidemic septicemia, a new infectious disease in fishes, which occurred among Cyprinoid fishes in China from 1989 were carried out. Fifteen bacterial strains were isolated from kidncy and liver of moribund fishes, including crucian carp (Carassius carassius), blunt-snont bream (Mygalobrama amblycephala) and silver carp(Hypothalmichthys molitrix), collected from six fishfarms in Jiangsu Province. All isolates shared following characteristics: Gram-negative rod, motile, oxidase positive, V-P positive, glucose, monnitol, salicin, arabinose, but not inositol fermentative, ornithine decarboxydase negative and resistant to novobiocin. These features indicated that the isolates belong to Aeromonas hydrophila. In addition, API-20E System was used to identify these isolates and reconfirmed the results.Pathogenicity test documented that the bacteria are the causitive agents of the discase. After experimentally infected by the bacteria via intrapcritoneal inoculation, crucian carp died of hemorrhagic septicemia, similar to naturally infected fish. The isolates were highly virulent (LD_(50)=5×10~3) and produced a hemolytic toxin.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1991年第4期87-91,共5页
Journal of Nanjing Agricultural University
关键词
鲁鱼
传染病
病原
单胞菌
Cyprinoid fishes
infectious discase
pathogen
Aeromonas hydrophila
