摘要
以嗜水气单胞菌(Aeromonashydrophila)灭活菌苗为免疫原,以平均体重为86 20g人工饲养的健康南方(Silu rusmeridionalis)为实验对象,通过腹腔注射免疫原(浓度为1 0×108/mL)免疫,剂量为0 2mL/尾,并分别在免疫后的第1、2、4、7、14、21、28天对实验鱼尾静脉采血,进行血液生理指标和血液中吞噬细胞活性的测定。结果显示,嗜水气单胞菌灭活菌苗(FKC)可以增加南方外周血液白细胞数量并引起不同种类白细胞组成比例的变化,提高吞噬细胞的吞噬活性。在免疫后第1、2、4、7、14、21、28天,南方外周血中白细胞数量呈明显上升趋势,其中嗜中性粒细胞、单核细胞、淋巴细胞变化趋势更为明显;而且吞噬细胞活性大为提高,吞噬百分比较对照组平均升高了17 66%,吞噬指数比对照组平均升高了1 16,在免疫后第4天吞噬百分比和吞噬指数均达到最高值,吞噬百分比为48 40%,吞噬指数为4 82;免疫后第21天,吞噬百分比和吞噬指数开始下降,但仍高于对照组。由此表明,使用疫苗后,鱼类非特异细胞免疫在初期(尤其是在第2～7天)明显增强,这是鱼用疫苗在免疫早期保护作用的主要机制之一。
Formalin-killed Aeromonas hydrophila cells (FKC) were used as immunogen to immunize healthy southern catfish (Silurus meridionalis) which were reared in the laboratory,the average body weight of which was 86.20g .After the safe-experiment and statistical test,each southern catfish was intraperitoneally injected with 0.2mL 1.0×10^(8 )cells/mL formalin-killed Aeromonas hydrophila. Then the haematological indices and activity of phagocytes in blood,which was collected from the tail veines of trial catfish,were investigated on the (1st),(2nd),(4th),(7th),(14th),(21st),(28th) days after immunization.The experiment showed that an increasing number of leucocytes and the activity of phagocytes as well as a change of percentage of leucocyte populations took place in the blood of southern catfish after the intraperitoneal injection of formalin-killed Aeromonas hydrophila.The leucocytes in the blood of trial catfish on the 1(st),2(nd),4(th),7(th),14(th),21(st),28(th) days after immunization increased drastically,especially the granulocytes,monocytes,lymphocytes.The dead Aeromonas hydrophila could increase the activity of phagocytes drastically.The phagocytic percentage and the phagocytic index in immunized group were 17.66% and 1.16 respectively,higher than those of their counterparts in the control group.The highest activity emerged on the 4(th) day after immunization,and the phagocytic percentage was 48.04% and phagocytic index was 4.82.Although it began to decrease from the 21(st) day after immunization,the activities of various phagocytes were still higher in immunized group than that in the control group.During the early period,especially from the (2nd) day to the (7th) day after injection of vaccines,the non-specific cell immunity of southern catfish increased prominently.The present study suggests that enhancing the non-specific cell immunity of fish be an important immune protective mechanism of fish vaccines.
出处
《中国水产科学》
CAS
CSCD
北大核心
2005年第3期275-280,共6页
Journal of Fishery Sciences of China
基金
重庆市应用基础项目资助(94-3997).
关键词
嗜水气单胞菌
疫苗
细胞免疫
南方鲇
血细胞
Aeromonas hydrophila
vaccine
cell immunity
Silurus meridionalis
blood cell
