摘要
目的 研究端粒酶在肺良、恶性病变和正常肺组织中表达的定位 ,以阐明端粒酶在上述疾病发病机制中的作用 ,以及在正常肺组织中的生物学意义。方法 对 3 8例肺癌、7例良性实质性病变及 3 5例病灶旁正常肺组织标本用核酸原位杂交和免疫组织化学技术对端粒酶逆转录酶 (hTERT)mRNA和蛋白质的定位表达进行了研究 ,同时与端粒酶活性进行了对比。结果 肺癌、良性实质性病变和病灶旁正常肺组织端粒酶活性阳性分别为 3 1 / 3 8、3 / 7、7/ 3 5(P <0 0 0 1 ) ;肺癌组织癌细胞、肺良性实质性病变中的淋巴细胞、纤维母细胞、巨噬细胞等以及病灶旁正常肺组织小气道、细支气管上皮的部分细胞和少部分肺泡上皮细胞hTERT蛋白有阳性表达 ,在有淋巴小结的肺组织其生发中心可见hTERT表达 ,多数巨噬细胞也有hTERT阳性表达。hTERTmRNA所表达的细胞与免疫组织化学结果类似。肺癌hTERT免疫染色平均吸光度比较 :鳞癌A值比腺癌略高 ,Ⅲ期比Ⅰ期、Ⅱ期稍高 ,分化程度越低A值也越高 ,但P >0 0 5。结论 ( 1 )端粒酶除了参与肺癌的发病机制外 ,也参与肺良性实质性病变发生发展。 ( 2 )端粒酶对于维持正常肺组织支气管上皮细胞、肺泡巨噬细胞和淋巴细胞的功能可能有重要的生物学意义。 ( 3 )端粒酶是一种增殖指标 ,而非肺癌的恶?
Objective To evaluate the diagnostic role of telomerase in benign and malignant lesion of lung and its biological significance in normal lung tiss ues. Methods Tissues studied consisted of 38 specimens of lung cancer, 35 adja cent non-neoplastic tissue and 7 benign parenchymatous lesion. Telomerase activ it y was determined using a semiquantitative polymerase chain reaction-based telom e ric repeat amplification protocol (TRAP) assay. Localization of telomerase rever se transcriptase(hTERT) expression was examined by in situ hybridization and imm unohistochemistry. Results Telomerase activity was detected in 31 out of 38 lun g cancer, 7 of 35 adjacent non-neoplastic lung tissue and 3 of 7 benign parench ym atous lesions (P<0.001). Both hTERT protein and mRNA were detected in cancer cel ls, lymphocytes, fibroblasts, macrophages in benign parenchymatous tissue, epith elial cells of small airways, bronchiole, alveoli as well as lymph nodule germin al center of the adjacent normal tissues. There was no significant difference in the expression intensity of hTERT protein among different kinds of cells. Th ere was a tendency that the expression intensity of hTERT protein was higher i n squamous carcinoma than those in adenocarcinoma and stronger at TNM stage Ⅲ wi th poorly differentiated than those at TNM stage Ⅰ～Ⅱ with well-differentiate d s quamous carcinomas and adenocarcinomas (P>0.05). Conclusions (1) Telomerase is involved in the pathogenesis of both lung cance r and benign parenchymatous lesion. (2) Telomerase expression may be of biolog ica l significance in maintaining the normal function of airway epithelial cells,alv eolar macrophage and lymphocytes. (3) It is suggested that telomerase might be used as a proliferative marker, rather than a specific malignant marker. False positive response may occur using telomerase as a diagnostic marker.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2001年第8期461-464,I002,共5页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
端粒酶
肺癌
端粒酶逆转录酶
发病机制
Telomerase
Lung cancer
Telomerase reve rse transcriptase
