摘要
目的 探讨非小细胞肺癌 (non smallcelllungcancer ,NSCLC)端粒酶活性 (telomeraseactivity ,TA)、端粒酶RNA(telomeraseRNA ,hTR)、端粒酶催化亚单位 (telomerasecatalyticsubunit,hTRT/hEST2 )编码基因的表达 ,彼此间相关性及其预后意义。方法 TRAP PCR(telomeraserepeatamplificationprotocolPCR)检测TA ,RT PCR检测hTR ,原位杂交检测hTRT/hEST2 mRNA表达。结果 非小细胞肺癌TA、hTR和hTRT/hEST2 mRNA阳性率分别为 6 8.4% (2 6 / 38)、5 5 .2 % (32 / 5 8)和 74.1% (4 3/ 5 8) ,TA与hTRT/hEST2 阳性相关 (r=0 .84,P =0 .0 1) ,与hTR无相关性 (r=0 .16 ,P =0 .2 3)。低分化以及有淋巴结或远处转移者TA及hTRT/hEST2 阳性率增高 ;TA及hTRT/hEST2 阳性组中位生存期 (10 .4个月 ,7.5个月 )低于阴性组 (13.5个月 ,14.7个月 ;P =0 .0 74,P =0 .0 1) ,hTR阳性组中位生存期 (12 .9个月 )略高于阴性组 (11.5个月 ,P =0 .2 3) ,仅hTRT/hEST2 阳性与阴性组生存期差异有显著性 ;多因素Cox回归分析hTRT/hEST2 对生存期有影响。结论 非小细胞肺癌TA、hTR和hTRT/hEST2 表达高于癌旁正常组织 ;hTRT/hEST2 够能反应TA活性 ,二者为NSCLC恶性表型增高的标志 ;hTRT/hEST2 具有独立的预后意义。
Objective To study telomerase activity(TA), telomerase RNA(hTR) and telomerase catalystic subunit (hTRT/hEST 2) code gene expression in non small cell lung cancer (NSCLC), their relationship and significance. Methods Telomerase repeat amplification protocol PCR(TRAP PCR) for TA, RT PCR for hTR and in situ hybridization for hTRT/hEST 2 mRNA expression were performed. Results Positive rates of TA, hTR and hTRT/hEST 2 were 68.4%(26/38), 51.7%(32/58) and 74.2%(43/58) in NSCLC. But, the adjacent and benign lung tissues were negative for expression of TA, hTR and hTRT/hEST 2. A statistically significant positive correlation between TA and hTRT/hEST 2 was observed( r =0.84, P =0.01), though there was no statistically significant positive association between TA and hTR ( r =0.16, P =0.23). The median survivals of TA and hTRT/hEST 2 positive groups (10.4 months,7.5 months) were shorter than the negative ones (13.5 months,14.7 months). However, only the hTRT/hEST 2 group was found to show statistical significance to be an independent prognostic factor by multivariate Cox regression analysis. Conclusion Telomerase activity, hTR expression and hTRT/hEST 2 are frequently detected at higher levels in NSCLC than the pericancerous normal tissues. There is a positive correlation between TA and hTRT/hEST 2, both of which are markers of poor magnificent phenotype of NSCLC. The hTRT/hEST 2 has an independent prognostic significance.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2001年第4期289-292,共4页
Chinese Journal of Oncology
基金
中国博士后科学基金资助项目(1998年)
关键词
肺肿瘤
癌
非小细胞肺
端粒酶
端粒酶RNA
端粒酶催化亚单位
Lung neoplasms
Carcinoma,non-small cell lung
Telomerase
Telomerase RNA
Telomerase catalytic subunit
