摘要
目的 :探讨再生障碍性贫血 (AA)患者的细胞免疫功能状况及其在AA发生、发展中的影响 ,为AA个体化治疗提供一定的依据。方法 :采用间接免疫荧光法、放射免疫测定法检测T细胞亚群、TNF α水平。采用微量甲基纤维素法研究了 2 9例AA患者粒一单系祖细胞 (CFU -GM )集落形成及其骨髓细胞和血清对正常CFU GM集落形成的影响 ,据此对AA进行发病机理分组。结果 :免疫介导组CD4 、CD8、HLA DR、CD4 /CD8、TNF α与正常组相比均具有显著差异性 ,干细胞缺陷组HLA DR明显高于正常组 (P <0 .0 5 ) ,CD4 /CD8比值显著低于正常组 (P <0 .0 1) ,而微环境缺陷组与正常组相比均无明显差异性。结论 :极大部分免疫介导组。
Objective: To explore the cellular immune condition of aplastic anemia (AA) patients and its influence on pathogenesis of AA. Methods: Phenotype of T lymphocytes was assayed with indirect immunofluorescence assay and serum TNF αlevel was determined with RIA in 29 cases. The bone marrow (BM) CFU GM colony growth and the influence of AA BM cells and serum on normal BM CFU GM were observed by methylcellulose micromethod. According to this, AA patients were classified. Results: Phenotype of T lymphocytes and serum TNF αlevel in immuno mediated AA were obviously different to normal controls. The percentage of HLA DR+cells In stem cell defective AA were more than that in normal controls (P<0.05), while the ratio of CD4/CD3 was lower (P<0.01). Conclusions: There were cellular immune abnormality in most of the immuno mediated AA, in some of the stem cell defective AA and in a few of microenvironmental defective AA.
出处
《江西医学院学报》
2000年第4期75-79,共5页
Acta Academiae Medicinae Jiangxi
基金
江西省卫生厅课题
