摘要
目的:观察候选新中药天升元口服液对免疫介导的再生障碍性贫血(immune-mediated aplastic anemia,IAA)模型小鼠的效应。方法:建立IAA小鼠模型,给予天升元口服液14 d后,检测外周血白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、红细胞比积(HCT)、血小板(PLT)、网织红细胞(RET)、股骨骨髓有核细胞(BMNC)和脾脏指数,并进行股骨和脾脏病理形态学观察。结果:模型对照组WBC,RBC,HGB,HCT,PLT,RET,BMNC和脾脏指数均较正常对照组显著下降(P<0.001);而天升元口服液(12.4,24.8 g.kg-1.d-1)能显著升高模型小鼠WBC(升幅分别达150%和133.3%),RBC(9.5%和37.1%),HGB(12.9%和44.5%),HCT(11.6%和28.4%),PLT(94.8%和252.3%),RET(115.4%和123.1%),BMNC(71.1%和95.6%)和脾脏指数(18.7%和33.7%),与模型组比较差异显著(P<0.05～0.001)。病理学检查显示天升元口服液明显增加骨髓造血细胞,降低脂肪细胞产生,减轻脾小体萎缩状态,促进脾小体髓外造血灶产生。结论:天升元口服液通过促进骨髓内外造血细胞增殖,改善造血功能,产生对IAA模型小鼠的治疗效应。
Objective：To observe the effects of Tianshengyuan oral solution（TSY）,a new candidate Chinese medicine,in mice with immunologically mediated aplastic anemia（IAA）.Method： Mouse models with IAA were developed and given TSY by gavage for 14 days.Then peripheral WBC,RBC,HGB,HCT,PLT,reticulocyte（RET）,femoral bone marrow nucleus cell（BMNC）,spleen index,and pathological features of femoral bone marrow and spleen tissues were examined.Result： The values of WBC,RBC,HGB,HCT,PLT,RET,BMNC,and spleen index in the model group were remarkably lower than those in the normal group（P0.001）.TSY（12.4,24.8 g·kg-1·d-1）could significantly increase the amount of WBC（at the ratio of 150.0% and 133.3%,respectively）,RBC（9.5% and 37.1%）,HGB（12.9% and 44.5%）,HCT（11.6% and 28.4%）,PLT（94.8% and 252.3%）,RET（115.4% and 123.1%）,BMNC（71.1% and 95.6%）and spleen index（18.7% and 33.7%）,compared with IAA model group（P0.05~0.001）.Furthermore,pathological examination for the femoral bone marrow and spleen tissues indicated that TSY could obviously increase in hematopoietic cells,decrease in fatty cells,alleviate the atrophy of the splenic corpuscles,and accelerate the growth of hematopoietic focuses in spleen.Conclusion： TSY could promote the proliferation of hematopoietic cells inside and outside bone marrow,improve the function of hematopoiesis,thus have a great potential for the treatment of mice with IAA.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第23期207-211,共5页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
再生障碍性贫血
天升元口服液
血细胞分析
骨髓
造血功能
aplastic anemia
Tianshengyuan oral solution
blood cell analysis
bone marrow
hematopoietic function
