摘要
在非繁殖季节,将17α-MT混入颗粒饵料,饲喂性腺未成熟的鲻鱼,对照组喂含酒精饵料,每日投饵一次,连续6周。研究结果表明:(1)实验后第二周,实验组有少数鱼轻压鱼腹可见少量精液流出,随后阳性率逐周提高,第五周达到高峰,其后持续较恒定水平。(2)激素处理组释精雄鱼的生殖指数大于不释精雄鱼,也大于对照组。(3)鲻鱼睾丸组织学切片看出,17α-MT处理的释精和不释精雄鱼精细管中各级生精细胞均处于生精活动的旺盛时期,而对照组精细管中充满精原细胞;另外,释精和不释精雄鱼的间质细胞的核的体积和数量都大于和多于对照组。因此,我们认为17αMT可刺激精原细胞增殖、精母细胞成熟分裂和精子形成。
Diets containing 17α methyltestosterone (17α MT) were fed to mullet during the early recrudescence of gonad in its reproductive cycle. The daily dosage of 17a MT per kg of body weight was 12.5 mg in experiment. Experimental fishes were fed with diet containing 17α MT every day for 6 weeks. The control group did not receive 17α MT. Results are as follows. (1) At the second week of the experiment, light pressure was applied to the abdomen and some amount of milt was observed being released in experimental group. Later the number of fishes releasing milt increased, reaching the peak at fifth week. (2) The gonadosomatic induces of the male fish releasing milt in the hormonally treated group were greater than the male fish releasing no milt and the control group. (3) According to the observation of the testicular section, the 17α MT might initiate the spermatogonia proliferation, spermatocyte meio-sis and spermiogenesis, and all the seminiferous tubule of (he male fishes releasing or not releasing milt of the treated group were active in spermatogenic activity, but all the seminiferous tubule of the control group were full of spermatogonia. On the other hand, the leydig cell nuclei were larger in the 17α MT treatment group than those of controls. This finding suggests the positive feedback of 17α MT on the pituitary-gonadal axis of the male mullet. Finally, the mechanism of 17α MT stimulatory spermatogenic activity was also discussed in the paper.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1989年第1期10-14,共5页
Oceanologia Et Limnologia Sinica
