摘要
硬头鳟早期发育过程中存在三种红血细胞核类型，圆形的幼体核（Ｌ核，在圆盘形的红血细胞内），椭圆形的成体核（Ａ核，在椭圆盘形的红血细胞内）和近椭圆形的未成熟的成体核（ＩｍＡ核，在近椭圆盘形的红血细胞内）。Ｌ核从胚胎早期发生（眼点期前后）至卵黄吸收完毕后（受精后６５日，孵出后３３日）消失，其间核直径逐渐减少，减幅达２９．７％。Ａ核从胚胎孵出时开始出现，至卵黄吸收完毕后，Ａ核基本取代其它类型核，其间核长短径持续发生变化。长径逐渐增加，短径逐渐减少，这种变化直至受精后９９日才稳定下来，此时Ａ核长径增幅为４３．１％．短径减幅为２２．３％．长短径比率由１．１１增至２．０４。ＩｍＡ核在胚胎孵出时开始出现，但比例很少，尺寸变化不大，在卵黄吸收完毕稍后（受精后７４日，孵出后４２日．）可忽略大计（基本上转变为Ａ核）。在胚胎孵出前，红血细胞核全为Ｌ核；在卵黄吸收期间，Ｌ，Ａ和ＩｍＡ３种混合存在，核大小及形状发生急剧变化，核类型迅速发生转换，最终全为Ａ核所取代．这种变化和Ｉｃｈｉｒｏ（１９７４）所描述的虹鳟红血细胞的变化基本一致。在印黄吸收完毕后（受精后７４日），Ａ核尺度相对稳定。Ｗｒｉｇｈｔ染色后在显微镜下观察，细胞核着色深，核形?
Yhere are tree types of erythrocyte nuclei exists in the early developing Steelhead trout,larval type (nucleus L. in the round disc-like erythrocyte),abuly type (nucleus A,in the elliptic disc-like adnly erythrocyte)abd immature adult type(nucleus ImA,in the immature erythrocyte). The round nucleus L ap=pears in the early embryonic development and remaims until swim-up sathe (65 days post-fertilization). They decrease gradually in diameter by 29. 7%. Tthe elliptic nucleus A appears from hatching(33 days post-fertilization) and dominate after swim-up stage. They coneynuously increase in majoraxis (a)by 43. 1 %and decreases in minor axis (b)22. 3% until 33 days post-swim-up (98 days postfertiization). The ratio a/bincreases accoriengly fron 1. 11to 2. 04. The nucleus ImA is presusde to be the precursor of nucleus A. they appear from hatching with a small proprtion.vary slightly,peak in numbers prior to swim-up and transit totally to nucleus A10days post - swim - up.Erythrocyte nuclei are larval type at prehatching. During yolk absortion. the three nucoear types esixt simulta-neously with a dramatic size variation and type transition. These changes keep until swim-up stage when if is dominated by the tytical elliptic abult erythrocyte nuclei, in respomdency fundermentally to the transition of erythrocytes im Rainbow trout described by Ichiro. Afterwatds nuclei A keep relativelt unchaigedin sizeabd can be deep -with Wright stain that can facilitate erythrocyte nuclear measurement for ploid determination. It is therefore, srggested to take sanples post -swim-up during the earlyde -velopment in Salmonids,such ac Steelhead Tuout.
出处
《水产学杂志》
CAS
1996年第1期1-6,共6页
Chinese Journal of Fisheries
关键词
硬头鳟
核长径
核短径
核转换
红血细胞核
早期发育
信性鉴定
Steelhead trout, nuclear major axis, nuclear minou axis, nuclear transition, erythrocyte nude - us
