摘要
目的 建立HLAI类基因分型技术并应用于骨髓配型。方法 采用序列特异性聚合酶链反应 (PCR SSP)进行HLA AB基因分型 ,并与HLAI类血清学分型 (微量淋巴细胞毒试验 )进行比较。结果 运用PCR SSP技术共对 2 2例需骨髓移植的病人和 48例供者进行基因分型 ,2例病人确认了HLA完全匹配的亲缘骨髓供者 ,其中 1例已成功进行了骨髓移植 ;基因分型和血清分型比较 ,43例结果完全一致 (6 1.4% ) ,血清分型错误率高达 38.6 % (2 7/ 70 )。结论 PCR SSP分型技术具有准确性高 ,简便快速等优点 。
Objective To develop the method for DNA typing of HLA class Igenome Methods HLA A and HLA B typing were performed by PCR SSP technique and a comparison of the typing results was carried out between serology and PCR SSP. Results Two recipients were compatible to HLA matching donors in 22 patients and 48 donors,and one of them succeeded in bone marrow transplantation(BMT).Of the 70 samples,43(61.4%)of the PCR SSP typings were in agreement with serology.However,there were 27 (38.6%)of 70 samples discrepancies between the serology typings and the molecular results.Conclusion The typing method of PCR SSP is accurate,simple and rapid,it might replace the serological testing for HLA
出处
《临床检验杂志》
CAS
CSCD
北大核心
2001年第5期272-273,共2页
Chinese Journal of Clinical Laboratory Science
