摘要
观察经颅重复性低频磁刺激 (rTMS)对大鼠纹状体FosB蛋白表达的影响。 6 OHDA单侧损毁纹状体边缘区 ,磁刺激器给予大鼠头部 1Hz ,10 0mT的重复性刺激 ,14d后用免疫组织化学ABC法检测纹状体FosB免疫阳性产物。rTMS后能够引起大鼠纹状体区域明显的FosB蛋白表达 ,这种表达广泛分布于尾壳核及苍白球 ,尤以腹侧纹状体及尾侧的壳核明显。 6 OHDA损毁后予以rTMS ,损毁侧FosB蛋白表达未出现减少 ,且尾壳核的背外侧部表达明显上调。对照组动物仅损毁侧有少量的FosB表达外 ,纹状体内未见FosB阳性标记。经颅重复性低频磁刺激能够激活纹状体内神经元 ,推测rTMS可能在增加多巴胺释放的同时 ,又能够激活多巴胺受体的活性 。
The effects of repetitive transcranial magneti c stimulation (rTMS)on FosB expression in rat striatum were observed. After unilat eral lesioning the marginal division of striatum by using 6 OHDA, animals were treated with 1 Hz, 100 mT transcranial magnetic stimulation for 14 d,and then Fo sB expressions in striatum were detected immunohistochemically. High FosB expres sions were induced in the striatum of experimental rats, especially in the rostr al ventral striatum and caudal putamen. There was an apparent upregulation of Fo sB expressions in the dorsalateral caudate putamen of 6 OHDA lesioned side afte r rTMS. In control animals, striatal cells did not express FosB like immunoreac tivity except few positive neurons appearing in 6 OHDA lesioned side. rTMS coul d activate the neurons in the striatum, which is presumed due to rTMS increase dopamine release and activate dopamine receptor. rTMS may play roles in treat ing Parkison's disease.
