摘要
对不施用任何肥料的浙江黄松土 (发育于河流沉积物母质的水稻田土 )、老黄筋泥田土 (发育于第四纪红壤母质的水稻田土 )、青紫泥田土 (发育于滨海盐土母质的象山青紫泥水稻土 )中甲烷氧化菌、产甲烷菌种群数量及甲烷排放量之间关系作了研究。结果表明 ,甲烷氧化菌种群数量范围在 1 0 6～ 1 0 8cfu/g干土之间 ,其变化在 2个数量级范围内。产甲烷细菌种群数量变化较大 ,其范围在 1 0 3～ 1 0 7cfu/g干土之间。水稻田土壤的甲烷排放量受到甲烷氧化菌、产甲烷菌种群数量及其活性及土壤理化性状的影响。提出了当水稻田土壤的甲烷氧化菌种群数量在 1 0 8cfu/g干土、产甲烷菌种群数量在1 0 5cfu/g干土时 ,水稻田土壤几乎没有多余的 CH4 气体排放这一甲烷形成与甲烷氧化两类群微生物之间的数量关系。甲烷排放量也与土壤砂粒 (<0 .0 2 mm的砂粒 )含量呈正相关性 ,土壤砂粒含量越高 。
Three types of paddy soil were sampled from different three sites of Zhejiang Province.Huangson rice field soil was developed from fluvo aquic soil matrix,old Huangjin rice field soil from the Quaternary red calay soil matrix and Qingzi rice field soil from coastal saline soil matrix,which were not applied any fertilizer.The bigger stones and larger fiber residues of plant were removed in the soil samples.15 kg of each soil sample was put into 13L plastic barrel and immerged over with water.Three plastic barrels of soil were replicated for each type of soil sample,two of which were planted rice plant and another one was not planted rice plant as control. Methane was measured using a 102G gas chromatograph with a hydrogen flame ionization detector under the following conditions:carrier:GDX 502,air:700 ml/min;H 2:40 ml/min;N 2:25 ml/min,room temperature:40℃.The appearance of the pure methane peak was at 17 sec.The pure methane gas,purchased from Beijing gases plant,was as the reference. The population of MOB was measured with the anaerobic roll tube method using Hungate's anaerobic technique but the medium was aerobically prepared and kept under aerobic condition in anaerobic tube with specific isobutyl rubber stopper to prevent the escape of methane gas.Each anaerobic tube with 4 5 ml melted aerobic medium was inoculated with different dilutions of soil sample and rolled,respectively,then was injected with 3 ml pure methane used as substrate for methane oxdizing bacteria and incubated at 30℃ for 7 days.The amount of colonies formed in the rolled tube with methane was counted after incubation. Medium for determination of methane oxidizing bacteria was composed of (g/L) KH 2PO 4 1 0,Na 2HPO 4·12H 2O 2 9,MgSO 4·7H 2O 0 32,(NH 4) 2SO 4 3 0,trace element solution 10 ml and distilled water 990ml,pH 6 8.The medium was aerobically prepared and 4 5ml of medium was added to each anaerobic tube,which was stopped with a specific rubber stopper. The population of MPB was determined using t
出处
《生态学报》
CAS
CSCD
北大核心
2001年第9期1498-1505,共8页
Acta Ecologica Sinica
基金
国家自然科学基金 (3 9870 0 3 3 )
中国科学院南京土壤研究所开放实验室 (971 5 3 1 )
国家教育部留学回国人员基金资助项目
