摘要
为探究全球气候变暖对青海湖湖滨湿地中甲烷氧化菌群落特征的影响,以青海湖湖滨湿地的鸟岛湿地观测站为研究对象,通过模拟增温实验设置开顶箱(open top chamber,OTC),利用高通量测序探究增温对湖滨湿地甲烷氧化菌群落结构及多样性的影响。湖滨湿地甲烷氧化菌群落的优势菌门是变形菌门(Proteobacteria)(84.50%);增温对湖滨湿地甲烷氧化菌群落的多样性指数没有显著差异影响(P>0.05),但对湖滨湿地甲烷氧化菌的属水平群落结构有显著影响(P<0.05)。LEfSe分析表明,增温与自然处理之间共存在33个差异菌群;增温使优势菌群甲基球菌科(Methylococcaceae)的相对丰度增高;增温使属水平的优势菌群甲基球菌属(Methylococcus)、甲基杆菌属(Methylobacter)、甲基单胞菌属(Methylomonas)的相对丰度升高,其中甲基单胞菌属的相对丰度显著升高,但硝化螺旋菌属(Nitrospira)和亚硝化螺旋菌属(Nitrosospira)相对丰度降低。湖滨湿地甲烷氧化菌群落功能基因组20个,其中大部分参与碳氮循环过程。整体而言,青海湖湖滨湿地群落结构对温度的响应更为敏感,部分菌群的相对丰度有显著性差异。
In order to explore the effects of global warming on the community characteristics of methanooxidizing bacteria in the lakeside wetland of Qinghai Lake,the bird island wetland observation station of Qinghai Lake was taken as the research object.Open top chamber(OTC)was set up through simulated warming experiment,and high-throughput sequencing was used to explore the response of community structure to warming and diversity of methanooxidizing bacteria in lakeside wetland.Proteobacteria(84.50%)were the dominant bacteria in the methane-oxidizing bacteria community in lakeside wetland.The diversity index of methanooxidizing bacteria community in lakeside wetland was not significantly affected by temperature increase(P>0.05),but had significant effects on the genus level community structure of methanooxidizing bacteria in lakeside wetland(P<0.05).LEfSe analysis showed that there were 33 different flora between warming and nature.The relative abundance of Methylococcaceae increased with temperature increase.And warming increased the relative abundance of dominant flora at genus level.The relative abundance of the three genera,Methylococcus,Methylobacter and Methylomonas increased significantly after the warming treatment.However,the relative abundance of Nitrospira and Nitrosospira decreased,while that of Methylomonas increased significantly.There were 20 functional gene groups of methanooxidizing bacteria in lakeside wetland,most of which were involved in carbon and nitrogen cycling.On the whole,the community structure of the lakeside wetland in Qinghai Lake was more sensitive to temperature,and the relative abundance of some microflora had significant differences.
作者
暴涵
章妮
陈克龙
Bao Han;Zhang Ni;Chen Kelong(College of Life Science,Qinghai Normal University,Xining,810008;Key Laboratory of Physical Geography and Environmental Process of Qinghai Province,Qinghai Normal University,Xining,810008;Key Laboratory of Ministry of Education for Surface Process and Ecological Conservation of the Qinghai-Tibet Plateau,Qinghai Normal University,Xining,810008)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2022年第9期1927-1937,共11页
Genomics and Applied Biology
基金
国家自然科学基金项目(41661023)
第二次青藏高原综合考察研究项目(2019QZKK0405)
青海省创新平台建设专项青海省自然地理与环境过程重点实验室项目(2020-ZJ-Y06)共同资助
关键词
甲烷氧化菌
气候变暖
碳氮循环
鸟岛湿地
Methanooxidizing bacteria
Climate warming
Carbon and nitrogen cycle
Bird island wetland
