摘要
目的 :研究人骨髓瘤细胞系Sko 0 0 7中IL 6信号途径活化与CD45诱导表达之间的关系。方法 :首先采用凝胶阻滞电泳 (electrophoreticmobilityshiftassay,EMSA)方法检测参与IL 6信号转导功能的转录因子STAT3和NF IL 6在Sko 0 0 7细胞中的诱导活化情况并确定该细胞中的IL 6信号转导途径 ;进而采用RT PCR和FACS方法检测CD45mRNA和蛋白在IL 6刺激作用下的诱导表达情况 ;最后采用以上两种方法观察CD45诱导表达与IL 6信号途径活化之间的关系。结果 :①两条主要的IL 6信号转导途径JAK/STAT和Ras/NF IL 6都能够在Sko 0 0 7细胞中诱导激活 ;②CD45mRNA和蛋白的表达量在IL 6刺激作用下明显增加 ;③STAT3反义表达质粒导入Sko 0 0 7细胞后JAK/STAT途径活化信号减弱 ,同时CD45mRNA诱导表达量下降。结论 :Sko 0 0 7细胞中JAK/STAT途径的诱导激活介导了CD45的诱导表达。
Objective:To investigate the relationship between “activation of IL 6 signal transduction pathways” and “induction of CD45 expression” in a myeloma cell line—Sko 007 by IL 6.Methods:Electrophoretic mobility shift assay(EMSA) was used to detect the activation of the transcription facotrs STAT3 and NF IL 6 by IL 6,which can represent the activation state of the two IL 6 signal transduction pathways respectively;Then the induction of CD45 mRNA and protein expression by IL 6 were detected by RT PCR and FACS.The effect of antisense expression plasmid of STAT3 pAT STAT3 on STAT3 activation and CD45 expression was shown by EMSA and RT PCR,respectively.Results:①Both two IL 6 signal transduction pathways JAK/STAT and Ras/NF IL 6 can be activated by IL 6 in Sko 007 cells.②The expression level of CD45 mRNA and protein is Sko 007 cells can be up regulated by IL 6.③The activation of JAK/STAT signal transduction pathway and induction of CD45 mRNA expression by IL 6 can be inhibited in pAT STAT3 transfected Sko 007 cells.Conclusion:IL 6 induced CD45 expression in Sko 007 cells in mediated by the activation of JAK/STAT signal transduction pathway.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第6期309-311,303,共4页
Chinese Journal of Immunology
基金
国家杰出青年科学基金资助!(No .3 992 5 0 19)
