摘要
目的 了解漏斗胸肋软骨基质形态学与组织化学特征。方法 对 19例漏斗胸和 14例同年龄儿童肋软骨基质的胶原进行电镜观察并对II型胶原进行免疫组化染色 ,对基质中蛋白多糖进行PAS和Safranin O染色。将所有染色结果进行图像分析。结果 与对照组相比 ,电镜下病变组肋软骨基质中胶原分布不均、排列紊乱 ;II型胶原免疫组化染色发现肋软骨深层II型胶原分布不均 ,但浅、深层II型胶原的染色强度没有明显改变 ;PAS和Safranin O染色发现基质中蛋白多糖的含量与分布均无明显改变。结论 漏斗胸肋软骨基质中蛋白多糖含量与分布及II型胶原的含量无明显改变 ,而基质中的胶原分布与排列异常 。
Objective: To evaluate the omrphological and histochemical properties of cartilage matrix of children with pectus excavatum(PE). Methods: The collagen of cartialge matrix of 19 PE and of 14 age matched children was examined by transmission electron microscopy(TEM) and immunohistochemical staining examination. Meanwhile, the proteoglycan was studied by PAS and Safranin O staining examination. All of the results of stanining were also analysed by the GT 2 model of image analysis software. Results: Compared with control group, although the intensity of type II collagen was intact, unequally distribution of collagen had been found by the TEM and immunohistochemical staining examination. The intensity and distribution of proteoglycan of PE group remained normal found by the PAS and Safranin O staining examination. Conclusion: Although the intensity and distribution of proteoglycan was intact, as well as intensity of collagen, the distribution of collagen in PE was abnormal, and this change may possibly be related to the defectiveness of costal cartialge of PE.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
2001年第3期159-161,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
关键词
漏斗胸
肋软骨
免疫组织化学
形态学
电镜
Pectus excavatum Costal cartilage Immuno histochemistry Morphology
