摘要
目的 构建新型人白细胞介素 2 ( 12 5 Ser)的表达载体 ,并在大肠杆菌中进行表达。方法 用PCR技术对天然人IL 2基因进行突变 ,并与表达载体 pET 11b连接 ,转化入大肠杆菌BL2 1中 ,用IPTG诱导表达。结果 将编码天然人IL 2的 12 5 Cys的密码子 (TGT)变成了编码 12 5 Ser的密码子 (TCT) ,在大肠杆菌中表达量达 2 7%。结论 构建了表达新型人IL 2 ( 12 5 Ser)的表达载体 ,并在大肠杆菌中获得高效表达 。
Objective To construct a vector expressing new-type interleukin-2(125-Ser)and expression in E coli.Methods The natural IL-2 gene was mutagenized by PCR.The mutated IL-2 gene was inserted into expression vector pET-11b,then transformed to E.coli BL 21 strain.Results 125-Cys codon(TGT)of natural IL-2 was changed to 125-Ser codon(TCT).The expressing level of new-type IL-2 reached 27% of total bacterial protein.Conclusions An expression vector of new-type IL-2 was established.And high level expression of IL-2 was obtained in E.coli.The work laid good foundation for the following renaturation and purification.
出处
《预防医学情报杂志》
CAS
2001年第4期227-228,共2页
Journal of Preventive Medicine Information
