摘要
为探讨铜缺乏对奶牛红细胞及组织细胞膜ATP活性的影响 ,在调查铜缺乏奶牛水土食物链微量元素状态的基础上 ,首次测试了病区 6头发病犊奶牛 (Ⅱ组 )及该地区 2 0头同年生临床健康奶牛 (IH组 ,血清铜低于 0 .5 6mg/kg)红细胞膜Na+ -K+ -ATP酶、Ca2 + -ATP酶及Mg2 + -ATP酶的活性 ,对 2 0头IH组奶牛进行为期 80d的补铜试验 (含铜40mg/kg干饲料 ) ,以 2 0头健区同年生健康奶犊牛作为对照 (HH组 ,全血铜大于 0 .85mg/L)。同时测试了剖杀后 6头病牛及健康牛组织细胞膜ATP酶的活性。结果表明 :Ⅱ组奶牛红细胞膜ATP酶活性显著低于HH组及IH组。补铜后40d ,IH组奶牛红细胞膜Na+ -K+ -ATP酶均显著升高。病牛肝、脾、肾、大脑、小脑、淋巴结等组织细胞膜Na+ -K+-ATP酶、Ca2 + -ATP酶活性显著低于对照组。结论 :铜缺乏较为严重地影响了奶牛红细胞及组织膜ATP酶的活性 ,抑制了这些组织的正常生理功能。
This study investigated the activities of Na+-K+-ATPase,Ca 2+ -ATPase and Mg 2+ -ATPase on the red cell membranes of 6 invalidcalves (ll Group) and 20 clinically healthy ones(IH Group,the serumcopper below 0.56 mg/L) at same age on condition that the status oftrace element on the soil-food chain were studied.The aim of this studywas to discuss the effect of copper deficiency on the activities of ATPase.20 IH Group calves were supplemented with 40 mg Cu/kg dry feedwhile 20 healthy calves in non-affected area were selected as control(HH Group,the content of whole blood copper is more then 0.85 mg/L).Theactivities of tissue membrane ATPase were also determined in 6 invalidcalves and healthy ones at same age.Results:The activities of ATPase ingroupll is significantlylower than that of group IH and HH.The activtiesof Na+-K+-ATPase and Ca 2+ -ATPase of red membrane of group IH were signifiantly improved after 40 days of copper supplementation,80 days later,the activities of all kinds of ATPase were significantly improved.The activities of Na+-K+-ATP?Ca 2+ -ATP on membrane of liver,spleen,kidney,brain,cerebrum and lymphoid of invalid calves were significantly lower than that of healthy ones.Conclusion:Copper deficiency had severe effect on the activities of the membrane ATPase of red cell and tissue in calves and restrained the normal physiological function of these tissue.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2001年第8期1-3,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
黑龙江省科委计划项目 (G95 130 332 )
