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注射含HCG和CPE的W/O/W复乳诱导日本鳗鲡性腺发育成熟的研究 被引量:8

Induction of gonadial development and maturation in Anguilla japonica
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摘要 :用含HCG及鲤脑垂体 (PG)匀浆液提取物 (CPE)的W /O/W复乳 ,对塘养雌鳗进行注射催熟实验。雌鳗平均GSI达 35.18% ,最高达 56.5% ,其催熟效果较常规注射方法 (平均GSI 2 7.9% ,最高 50 .4 % )好。用含LHRH A、HCG、CPE的复乳和DOM生理盐水混悬剂对部分达到性成熟的鳗鲡进行催产 ,用W /O/W复乳诱导成熟的鳗鲡 ,排卵率为 75% ;用常规方法催熟的鳗鲡排卵率为 55%。注射复乳制剂后 ,鳗鲡血清GtH含量可较长时间的保持较高水平 ,血清GtH的变化较常规注射方法缓和。注射乳剂还可使鳗鲡脑垂体GtH含量升高。 Female cultivated eel (Anguilla japonica), body weight 330~680 g, body length 62~67 cm, were employed without being fed during the experiment with water temperature 14~25℃, salinity 30~34 and natural light intensity. In the gonadial maturation induction test, by injecting the W/O/W (water oil water) multiple emulsion 12 times at 7 d intervals, containing HCG & CPE (carp pituitary extraction), the average GSI of the eel increased to 35.1% with the highest 56.5%. While the average GSI in the groups injected with routine HCG & CPE is 27.9% with the highest 50.4%, lower than that in the W/O/W groups. In the ovulation induction test the female eel were induced by injecting the W/O/W multiple emulsion (containing LHRH A, HCG and CPE) and DOM. The ovulating rate of the eel in W/O/W groups was more than those in routine HCG & CPE groups. The serum GtH levels in W/O/W groups remained significantly higher than those in routine groups.
出处 《中国水产科学》 CAS CSCD 北大核心 2001年第2期44-47,共4页 Journal of Fishery Sciences of China
基金 国家自然科学基金资助项目 (39770 10 1) 国家"九五"攻关专题项目 (96 0 0 8 0 1 0 1 0 1)
关键词 水/油/水复乳 日本鳗鲡 性腺发育 催熟 催产 人工繁殖 脑垂体 HCG CPE W/O/W multiple emulsion Anguilla japonica gonad development maturation induction spawning induction
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  • 1Yu K L,Gen Comp Endocrinol,1987年,51卷,2期,234页 被引量:1
  • 2林浩然,水生生物学报,1987年,11卷,3期,320页 被引量:1
  • 3王义强,水产学报,1980年,4卷,2期,147页 被引量:1

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