摘要
在毛细管电泳仪上对１２种β－受体阻滞剂和类似物的光学对映体进行了分离，研究了它们的拆分条件。使用 ０．１ｍｏｌ／Ｌ， ｐＨ ３．０的磷酸－三乙醇胺缓冲液，２０ｋＶ电压， １２～３６ｍｍｏｌ／Ｌ羟丙基－β－环糊精作为手性识别试剂。其中 １１种β－受体阻滞剂及其类似物以及苯乙醇胺、胺酵素在 ５０ ｃｍ普通毛细管上获得了较好的手性分离效果，普罗勒沙的对映体在本条件下未获分离。同时发现，芳香环上取代基位置对迁移时间的影响有一定规律。
Chiral separation conditions of 12 kinds of β-blockers and β-blocker analogues were investigated by capillary electrophoresis. 11 of them, phenylethnolamine and clenbuterol were separated successfully under optimum conditions: 0. 1 mol/L, pH 3 .0 phosphate - trihydroxyethylamine buffer and proper concentration of hydroxypropyl-β cyclodextrin (12 - 36 mol/L) at 20 kV voltage in 50 cm unmodified capillaries, but enantiomers of pronethalol was not separated. It was also found that the position of substituting group took effect on migration time.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第6期671-675,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助课题(No.29790128)
