摘要
高灵敏的由柠檬酸钠稳定的金纳米粒子通过共振瑞利散射法(RRS)和比色法测定普萘洛尔。利用柠檬酸钠作为还原剂,成功合成了表面带负电的金纳米粒子。在pH 4.6的BR缓冲溶液中,普萘洛尔由于质子化带正电,因此金纳米粒子与普萘洛尔可以通过静电作用结合,从而使金纳米粒子聚集,导致体系的RRS信号增强,并且伴随溶液的颜色由酒红色变成紫色。一种高灵敏的RRS和比色法因此建立起来用于测定普萘洛尔,两种方法对应的线性范围分别为0.2–5.2和0.4–4.4μg·mL-1。但是将该方法用于其他洛尔类药物(比索洛尔、阿替洛尔、美托洛尔和阿罗洛尔)的测定,发现这些洛尔类药物对柠檬酸钠修饰的金纳米粒子探针没有如普萘洛尔般的响应,因此可以用此法识别洛尔类药物混合物中的普萘洛尔。
In this work,a determination of propranolol in real samples based on highly sensitive citrate anion-cappedgold nanoparticles(AuNPs)was developedby using resonance Rayleigh scattering(RRS)and colorimetry.By sodium citrate reduction method,citrate anions self-assembled on the surface of AuNPs to form supermolecular complex anions;and on the other hand,in BRbuffer solution(pH 4.6),propranolol was positively charged and could bind with AuNPs to form larger aggregates through electrostatic force and hydrophobic effects,which resulted in an remarkable enhancement of RRS intensity and a color change of AuNPs solution from red to blue via purple at the same time.Thus a highly sensitive RRS and colorimetric assay for detecting propranolol has been developed with the linear range of 0.2~5.2and 0.4~4.4μg·mL-1,respectively.This purobe hadμgood selectivity to propranolol and no response to other similar drugs,such as bisoprolol,atenolol,metoprolol and arotinolol.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2016年第S1期439-440,共2页
Spectroscopy and Spectral Analysis
关键词
金纳米
共振瑞利散射
比色法
普萘洛尔
Gold nanoparticles
Resonance Rayleigh scattering
Colorimetic
Propranolol
