摘要
用核酸原位杂交和用免疫组织化学法检测了 6例豚鼠正常肺组织、10~ 18例癌旁正常肺组织和 14例正常人外周血端粒酶逆转录酶 ( TERT)亚基的 m RNA和相应蛋白质表达。结果显示 ,豚鼠和人体正常肺组织支气管上皮细胞 ,肺泡巨噬细胞和少数肺泡上皮细胞有 TERT m RNA和蛋白质表达。正常人外周血淋巴细胞中 TERT原位杂交阳性表达细胞数为 ( 17.2 6± 8.5 7) % ,免疫组织化学阳性细胞表达数为 ( 2 8.10± 16.78) %。提示 :1正常肺组织支气管上皮细胞、肺泡巨噬细胞、少数肺泡上皮细胞和外周血淋巴细胞有 TERT m RNA和蛋白质表达 ;2正常肺组织中上述细胞TERT m RNA和蛋白质的表达在生理状况下对于保证支气管上皮和肺泡上皮细胞再生 ,维持上皮组织的完整性可能起着重要的作用 ,同时有利于肺泡巨噬细胞发挥其肺内“卫士”功能 ;3淋巴细胞
In situ hybridization and immunohistostaining methods were employed to detect the expression of mRNA and protein of telomerase reverse transcriptase in normal lung tissue of 6 guinea pigs, adjacent normal tissues of 10-18 cases of carcinoma and circulating lymphocytesof 14 healthy subjects The results showed that TERT mRNA and protein expression were detectable in epithelial cells of bronchi, pulmonary alveolar epithelial cells and alveolar macrophages of normal lung tissue in guinea pig and human body In healthy subjects, the circulating lymphocytes positive for TERT expression were (17 26±8 57) % by in situ hybridization and (28 10±16 78) %, respectively It was suggested that the expression of TERT mRNA and protein in some actively regenerating normal pulmonary cells might play a critical physiological role in keeping regeneration and integrality of airway epithelial tissue and pulmonary alveolar epithelium It was also very important for alveolar macrophage to play as 'safeguard' function in normal lung The expression of TERT in circulating lymphocytes might play a critical role in maintaining normal human immunological function
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第2期112-115,共4页
Acta Universitatis Medicinae Tongji