摘要
目的 :观察兔股动脉缩窄与压力变化的关系 ,探讨用球囊损伤的方法建立兔股动脉血栓形成模型。方法 :新西兰兔 10只 ,用细尼龙线逐渐缩窄兔右股动脉至完全闭塞 ,记录缩窄程度与股动脉压力的变化。继之经右股动脉置入 2 .5mm×2 0 .0mm球囊剥脱血管内皮 ,抽出球囊后监测股动脉压力变化。球囊损伤前后取血检测血小板计数。对部分动物在血栓形成前后行股动脉造影。最后取受损节段血管作病理检查。结果 :①兔右侧股动脉缩窄 90 %时 ,其收缩压、脉压显著下降 ;进一步缩窄达 10 0 %时 ,脉压降为 0 ,同时舒张压、平均压也显著下降。②右侧股动脉收缩压、脉压在球囊损伤后 5min即显著下降 ;至损伤后 90min ,脉压降至 0 ,同时舒张压、平均压亦显著下降。③动脉造影及病理组织检查均证实球囊损伤后股动脉血栓形成 ,管腔完全闭塞。④球囊损伤后 2h血小板计数显著下降。结论 :球囊损伤可制成稳定、可靠的兔股动脉血栓形成模型 ,这在进行各种溶栓剂的药代动力学和药效动力学研究时具有实用价值。
Objective:To observe the relationship between the grade of constriction and blood pressures of the rabbit femoral artery, and construct an animal model of the femoral artery thrombosis by balloon injury. Methods:In 10 New Zealand rabbits, the right femoral arteries were gradually narrowed by nylon thread till complete occlusion. The grade of constriction and corresponding femoral artery blood pressures were recorded. Then a 2.5 mm×20.0 mm balloon was put into the right femoral artery to induce endothelial denudation. The femoral artery blood pressure was monitored after the balloon was pulled out. Blood samples were collected to determine the platelet quantity pre- and post-balloon-injury. The femoral artery angiography was performed in a few rabbits before and after the injury. All the injured arteries were segmented for the pathological study at last. Results:①Systolic blood pressure and pulse pressure dropped in the rabbit femoral artery while the artery was constricted by 90%. Moreover, the pulse pressure reduced to zero when the artery was completely occluded, with a simultaneous drop of the diastolic blood pressure and mean blood pressure. ②The systolic blood pressure and pulse pressure of femoral artery dropped significantly 5 minutes later of the balloon injury, and the pulse pressure reduced to zero 90 minutes after the injury, with a significant decrease of the diastolic blood pressure and mean blood pressure. ③Both the femoral artery angiography and histological examination showed that the thrombus was created in the femoral artery after balloon injury and the arterial lumen was occluded completely. ④The quantity of platelet significantly decreased 2 hours later of the balloon injury. Conclusion:Balloon injury could make a stable and reliable thrombus model of the femoral artery in rabbits, which will be very useful to the pharmacokinetic and pharmacodynamic study of the thrombolytic agents.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第4期277-280,F003,共5页
Journal of Nanjing Medical University(Natural Sciences)
关键词
动物模型
球囊损伤
股动脉血栓形成
femoral artery
balloon
thrombosis
animal model
rabbits
