摘要
目的:探讨99Tcm标记重组改良型人膜联蛋白V(H-Annexin V)在健康小鼠体内的生物分布及在健康家兔体内的动态显像表现。方法:以99Tcm直接标记H-Annexin V,然后测定标记率,并计算其比活度。研究99Tcm-H-Annexin V于1,10,30,60,120和240min在健康小鼠体内的生物分布特性;通过SPECT显像,运用感兴趣区(ROI)时间-放射性曲线分析,观察在240min内99Tcm-H-Annexin V在健康家兔体内的动态分布变化。结果:99Tcm-H-Annexin V标记率为(96.80±0.35)%,比活度为(12.30±0.06)TBq/mmol。小鼠血液放射性清除迅速,肾脏排泄较快,其余组织器官放射性均随时间逐渐降低,而脑放射性水平始终最低;家兔各器官放射性均随时间逐渐下降,与同一时间肾、心、肝相比,肺、胃、肌肉放射性呈低水平。结论:99Tcm-H-Annexin V制备方法简便,标记率高,体内稳定性好,具有比较理想并符合实际的动物体内动力学表现。
Objective： To investigate the biodistribution of ^99Tc^m-H-Annexin V in normal mice and rabbit. Methods： H-Annexin V was labeled directly with 9Wcm. The labeling rate was determined and the specific activity was calculated. The biodistrbution of ^99Tc^m-H-Annexin V was studied at 1, 10, 30, 60, 120 and 240min after injection in normal mice. The scintigraphy of ^99Tc^m-H-Annexin V was performed in normal rabbit for 240min, combining the region of interest（ROI） time-activity curve analysis. Results： The radiolabeling rate and specific activity of ^99Tc^m-H-Annexin V were （96.80±0.35）% and （12.30±0.06）TBq/mmol respectively. Biodistribution in mice showed rapid blood and renal clearance with gradually excreted in other organs or tissues including heart, liver, lungs, and muscle. Low uptake at brain was also observed throughout the whole course of the studies. In rabbit, rapid uptake and clearance in the genito-urinary system with gradually decreased uptake with time in other organs or tissues was observed. Conclusion： ^99Tc^m-H-Annexin V is an easily prepared and labeled compound with high labeling efficiency and stability.
出处
《中国临床医学影像杂志》
CAS
北大核心
2008年第1期56-58,共3页
Journal of China Clinic Medical Imaging
关键词
膜联蛋白A5
同位素标记
锝
动物实验
药代动力学
Annexin A5
Isotope labeling
Technetium
Animal experimentation
Phannacokinetics
