摘要
用表达型质粒载体pSPORT 1构建了经白粉菌 (Erysiphegraminis)诱导 48h和未经诱导的小麦 簇毛麦 6VS/ 6AL易位系(Triticumaestivum Haynaldiavillosatranslocationline)叶片cDNA文库各一个。文库宿主菌为E .coliDH10B。非诱导文库包含约 5 0万个重组cDNA克隆 ,平均插入片段为 1 2 5kb ,主要分布在 40 0bp～ 2 2kb。诱导文库包含约 30万个重组cDNA克隆 ,平均插入片段 1 2kb。用克隆的病程相关基因 (pathogenesisrelatedgene) 小麦类甜蛋白基因pWIR作探针 ,进行杂交筛库 ,杂交信号明显 。
Two cDNA libraries of Triticum aestivum Haynaldia villosa 6VS/6AL translocation line were constructed using mRNA from leaves of its plants induced and non induced with Erysiphe graminis.An expression plasmid p SPORT 1 introduced into E.coli DH10B was used as cloning vector.The cDNA library of pathogen induced translocation line comprised of about 300 000 recombinant cDNA clones with average insert size of 1 2 kb,and that of non induced translocation line consisted of aproximately 500 000 recombinant cDNA clones with average insert size of 1 25 kb,ranging basically from 0 4 to 2 2 kb.The library was successfully screened with a wheat thaumatin like protein gene pWIR as the probe.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2001年第1期5-8,共4页
Journal of Nanjing Agricultural University
基金
86 3计划资助项目! (Z 17 0 4 0 1)
