摘要
螺旋霉素链霉菌生物合成螺旋霉素的过程受许多酶的调控作用。酰基激酶和酰基 Co A合成酶是螺旋霉素内酯环合成的重要酶。实验测定了它们的酶活趋势和酶的影响因子。结果表明 ,酰基激酶和酰基 Co A合成酶都有两个活力峰 ,前者活性集中在发酵中前期 ,后者活性集中在发酵中后期。实验发现 Co2 + 、Mn2 + 对酰基激酶和酰基 Co A合成酶有较强的激活作用 ,Cu2 + 对酰基激酶有抑制作用 ,但对酰基 Co A合成酶有很强的激活作用。在发酵中期向摇瓶中补加二价阳离子比在发酵初期加入有更明显的激活作用 ,平均效价最高提高了 31 %
The biosynthes of spiramycin by Streptomyces ambofaciens was affected by the enzymatic system including acyl CoA synthetases and acylkinases. The activity and the influencing factors of those two enzymes have been studied. Both acylkinase and acyl CoA synthetase have two activity peaks. The former was only present in the prophase and the metaphase; the latter was synthesized during the metaphase and the anaphase. The results indicated that acylkinase and acyl CoA synthetase could be activated by Co 2+ and Mn 2+ while Cu 2+ could inhibit the former but activate the latter. Adding the influencing factors in the metaphase have a distinct activation than in the prophase. The maximum of improvement of average production was 31%.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2001年第3期251-253,共3页
Journal of East China University of Science and Technology
