摘要
目的建立一种简便而经济的大鼠肝脏贮脂细胞的分离方法。方法采用链霉蛋白酶和Ⅳ型胶原酶循环灌注大鼠 肝脏后，以１１％Nycodenz密度梯度离心，分离肝脏贮脂细胞。结果细胞得率为２×１０～３×１０个／只，活力在９５％以上， 纯度超过９０％。结论本法简化了肝脏贮脂细胞的分离过程，使之既简便又经济，且细胞得率也较高。
Objective To establish a simple and economic method for isolating rat hepatic lipocytes. Method Perfusion of the rat liver with pronase E and collagenase in succession was performed in cycles, and the hepatic lipocytes were isolated and purified by digesting the rat liver with DNase in the presence of the above 3 enzymes, followed by 11% Necodenz density gradient centrifugation. The resulted lipocytes were subjected to routine identification procedures and viability assessment. Results The yield of the lipocytes from each rat was 2×10 to 3×l0, with viability over 95% and purity above 90%. Conclusion The method adopted in this study is simple, economic and highly productive.
出处
《第一军医大学学报》
CSCD
北大核心
2001年第4期304-306,共3页
Journal of First Military Medical University
基金
国家自然科学基金!(39870331)
