摘要
目的：探讨细胞因子对小鼠α２（Ｉ）型前胶原基因上游启动子活性的影响。方法：以小鼠α２（Ｉ）型前胶原基因启动子（－２ｋｂ～＋５４ｂｐ）为靶序列，将它与氯霉素乙酰基转移酶（ＣＡＴ）报告基因组成的重组体ｐＡＺ１００９用脂质体法转染至胶原产生细胞ＮＩＨ／３Ｔ３细胞，讨论ＩＦＮα、ＩＦＮγ、ＴＮＦα对小鼠α２（Ｉ）型前胶原基因上游启动子活性的影响。结果：ＴＮＦα抑制小鼠α２（Ｉ）型前胶原基因启动子活性，ＴＮＦα分别与ＩＦＮγ、ＩＦＮα联合应用能加强这种抑制作用。结论：ＴＮＦα分别与ＩＦＮγ、ＩＦＮα联合应用能加强抑制作用，这可能与ＩＦＮγ、ＩＦＮα诱导ＴＮＦα受体表达，从而加强ＴＮＦα抑制作用有关，而ＩＦＮγ抑制胶原蛋白合成作用与该序列无直接相关。
Objective: To study the effect of cytokines on promoter activity of 2(I) procollagen gene. Methods: This study focused on the promoter sequence -2 kb +54 bp of mouse 2(I) procollagen gene and the effect of TNF, IFN, IFN on promoter activity. The construction pAZ1009 containing this sequence and CAT as reporter gene was transfected to cytokinestreated NIH/3T3 cells with liposomal transfection method. Cytokines treatment was continued for another 24 hours after transfection. Results: The study reveals that TNF inhibits promoter activity of mouse 2(I) procollagen gene, this inhibition effect is even stronger when TNF was used in combination with IFN or IFN. Conclusion: A potential explanation for this synergism is provided by the finding that IFN, IFN can increase the number of TNF receptor, thus making these cells more susceptible to TNF modulation. The inhibition effect of IFN on procollagen production seems have no direct relation with the promoter sequence.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第7期296-298,共3页
Chinese Journal of Immunology
基金
国家自然科学基金
