摘要
目的 :研究老年慢性肺心病 (CPHD)患者不同病期外周血淋巴细胞 (PBL)凋亡的变化 ,以及肿瘤坏死因子(TNF -α)与一氧化氮 (NO)在发病中的作用 ,选取CPHD不同时期的患者 30例与 15例正常人进行比较 ,并探讨其临床意义。方法 :采用DNA断端标记法 (TdT法 )、ELISA法及griess法分别检测CPHD患者血淋巴细胞凋亡率的变化以及TNF、NO的水平。结果 :患者急性加重期的PBL凋亡率 (33 .12± 7.80 % )明显高于对照组 8.32± 1.43 % (P <0 .0 1)和缓解期 8.77± 2 .17% (P <0 .0 1) ,TNF、NO的血浆浓度随病情变化而变化。结论 :老年CPHD患者PBL凋亡率TNF、NO与病情有关。
Objective:To study the apoptosis of peripheral blood lymphocytes (PBL) and serum levels of tumor necrosis factor (TNF-α) and nitric oxide (NO) in 30 aged patients with chronic pulmonary heart disease (CPHD) during different periods and 15 normal subjects.Methods:In situ terminal deoxynuleotidyl transferase-mediated nick-end labeling (TDT) method and ELISA method and griess method were used.Results:Apoptotic percent ages of the PBL in patients with acute exacerbation stage (33.12±7.80%) were more than those of the PBL in controls (8.32±1.43%) (P<0.01) and remission stage (8.77±2.17%)(P<0.01) and serum levels of TNF-α and NO in patients with acute exacerbation stage (1.87±0.33ug/L;150.05±44.07umol/L) were more than controls (0.67±0.21ug/L,33.69±12.08umol/L)and remission stage (1.16±0.28ug/L,36.45±14.97umol/L).Conclusions:Apoptotic percent of PBL and serum.levels of TNF-α and NO related to the stage of aged patients with CPHD.
出处
《中国现代医学杂志》
CAS
CSCD
2001年第4期42-43,45,共3页
China Journal of Modern Medicine
关键词
慢性肺原性心脏病
细胞凋亡
肿瘤坏死因子
一氧化氮
Chronic Pulmonary Heart Disease
Lymphocytes Apoptosis Tumor Necrosis Factor
Nitric Oxide
