摘要
Lysine-rich protein gene (lys) was cloned from Psophocarpus tetragonolobus (L.) DC. A plant expression plasmid was constructed and lys gene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter. The plasmid was introduced into rice embryogenic calli by microprojectile bombardment. The regenerated fertile plants were obtained by effective selection for hygromycin B resistance. Genomic PCR and Southern blotting analyses showed that the lys gene has been integrated into rice genome. Simultaneously, the results of GUS histochemical assay demonstrated that gus report gene is also expressed in leaves, stems and roots of the transgenic rice plants. Data analysis showed that lysine content in most of the 11 transgenic plants is differently improved, and in one of them increased by 16.04%.
构建了一个植物高效表达质粒 ,使来源于四棱豆 (Psophocarpustetragonolobus (L .)DC)的高赖氨酸蛋白基因(lys)受控于单子叶植物ubiqutin强启动子下表达。用基因枪法将其导入水稻 (OryzasativaL .)幼胚诱导的愈伤组织 ,经潮霉素抗性筛选 ,得到可育的再生植株。经PCR和Southernblotting检测 ,表明该基因已整合到水稻的基因组中。GUS组织化学染色表明转基因水稻植株的叶、茎和根中均有gus基因的表达。测定 11株转基因水稻叶片中赖氨酸含量 ,大部分植株有不同程度的提高 ,最高幅度为 16 .0 4%。
